Phosphorylated Protein Analysis Service

Introduction

Phosphorylation is one of the most important post-translational modifications in cellular activities, and it is involved in the regulation of almost all cellular activities, including cell proliferation, differentiation, and apoptosis. Phosphorylation modification has the characteristics of simplicity, flexibility and reversibility, and the availability of ATP, a donor of phosphate groups, is a common regulatory means in eukaryotic cells and plays a role in various cellular activities, including glycolysis, protein -protein interactions, protein degradation, enzyme inhibition, homeostasis, etc. Protein phosphorylation is the reversible process of attaching phosphate groups to proteins catalyzed by enzymes, mainly on serine, threonine or tyrosine residues. Notably, mass spectrometry (MS) has revolutionized signal analysis by allowing rapid identification of phosphorylation sites in a precise and sensitive manner and is a powerful tool for phosphorylation analysis.

Fig. 1. Functional significance of protein phosphorylation. (Roux P P, et al., 2013)

Our Services

Creative Proteomics provides a high-quality and highly sensitive phosphoproteome analysis platform to determine phosphorylation and phosphorylation sites by applying mass spectrometry to your phosphopeptides.

At Creative Proteomics, we can perform large-scale analysis of complex samples, which often requires enrichment of peptides prior to mass spectrometry analysis. Our highly skilled personnels provide various enrichment methods for phosphorylated peptides according to experimental needs, including TiO₂ and IMAC enrichment, solid-phase metal affinity chromatography (IMAC) enrichment, and phosphorylated antibody enrichment.

Based on our well-established phosphoproteome analysis platform, Creative Proteomics can provide an accurate one-stop phosphoprotein analysis service package, including protein extraction, digestion, phosphopeptide enrichment, peptide separation, MS analysis and bioinformatics analyze.

Here, we can provide but not limited to:

• Phosphorylation Analysis
• Phosphopeptide Enrichment
• Phosphorylation Site Identification
• Phosphorylation Site Mapping
• Protein-Protein Interaction Analysis

Advantages of Our Services

Our analytical services strictly follow ICH Q6B guidelines, and a high-quality phosphorylated protein analysis services are provided through high-performance mass spectrometry (MS) and liquid chromatography systems, etc., which ensures that our phosphorylated protein assays have the following attractive features.

• High precision: A high-throughput and high-precision detection results can be guaranteed using phosphorylated protein enrichment and sensitive MS analysis.
• Extreme accuracy: Combined with iTRAQ/TMT labeling to reduce within-sample variability, yield consistent and accurate phosphorylation identification.
• Eperienced professionals: Our experienced team of scientists and analysis help with phosphorylated protein analysis.
• Customized service: Customized service based on sample type and project to meet your special requirements.
• Advanced mass spectrometry platform: It ensures reliable and reproducible results.
• Detailed report: You will receive an easy-to-understand report describing the data analysis and results step by step, as well as a folder with all tables and figures.

Sample Requirements

1. Tissue samples: Plant tissue samples > 200 mg, blood samples > 1 mL (with EDTA for anticoagulation of plasma), serum > 0.5 mL, urine> 2 mL, animal tissue samples > 1 g, cell samples > 5X10⁷ cells, and microorganisms > 200 mg (dry weigh).
2. Protein samples > 1 mg.

Thanks to our powerful mass spectrometry sequencing platform, Creative Proteomics provides a one-stop hosphorylated protein analysis service. Our experienced scientists work with you to develop tailor-made analytical solutions. All you need to do is send us your samples, communicate your research goals with our experts, and we'll go through the next steps and provide you with a full technical report. Please feel free to contact us with any questions regarding hosphorylated protein analysis.

References

1. Roux P P, Thibault P. (2013) The coming of age of phosphoproteomics--from large data sets to inference of protein functions. Mol Cell Proteomics. 12(12):3453-3464.
2. Dephoure N, Gould K L, Gygi S P. et al. (2013) Mapping and analysis of phosphorylation sites: a quick guide for cell biologists. Mol Biol Cell. 24(5):535-542.