Comparative Proteomics in Venom

Comparative proteomics is also called differential proteomics, and its main content is to screen and identify the expression differences of the proteome under different conditions or between different types of samples. The purpose of comparative proteomics is to analyze the changes in the proteome in response to development, disease, or the environment. Because the venom is a mixture of many components, its composition can exhibit plasticity. Different diet composition, environmental factors, and external stimuli can cause mutations, and their proteome will change accordingly. Therefore, the study of differential proteome helps to directly understand the nature of life activities.

Common Methods

At present, common methods for analyzing differential proteomics include two-dimensional electrophoresis and methods related to mass spectrometry. With the development of mass spectrometry and mass spectrometry-related technologies (such as labeling technology, mass spectrometry data acquisition technology), mass spectrometry has the advantages of high resolution, high sensitivity and high throughput in proteome analysis, and can perform qualitative and quantitative analysis of proteome. For example, methods such as SILAC, TMT, iTRAQ, etc. can perform label detection on different samples to achieve relative or absolute quantification of the protein group, and the relevant information of the differential protein group can be obtained by analyzing the detection data.

Two-step Process

We provide a two-step process in which the proteins in the cell extract are first fractionated to reduce the complexity of the sample, and then the proteins are identified by mass spectrometry. Two-dimensional electrophoresis (2DE) is a long-term standard for protein separation, but its reproducibility is poor and sensitivity is limited. In order to overcome the limitations of reproducibility and sensitivity, differential gel electrophoresis (DIGE) was developed, in which two protein samples were labeled with different fluorescent dyes and then electrophoresed together on the same 2DE gel.

In general, DIGE is much more sensitive and reliable and offers a broader dynamic range in comparison with classical gel staining. Additionally, it shows better reproducibility of the results, due to the fact that several samples may be separated on the same gel. The possibility of using an internal standard makes matching between repetitive gels easier and allows for obtaining better accuracy during quantitative analysis. The fact that a lower number of gel repeats is necessary for statistical purposes is also an asset.

Creative Proteomics can provide differential proteomics services to biotech companies, pharmaceutical companies, research institutes and universities. We have many years of experience in proteomics analysis, which allows us to provide reliable and accurate results at a competitive price and ensure greater efficiency.

Please contact us for more information.