Venom Glycomics Services

Creative Proteomics is based on the Obitrap Fusion Lumos, a high-resolution mass spectrometer, and uses Byonic software, which contains almost all glycoforms, to perform glycosylation analysis of peptides and proteins from venoms or poisonous species. The glycosylation sites, N-Glycan /O-Glycan types, and glycan composition of glycosylation sites can be determined for peptides or protein samples. Provide data support for the follow-up venom as the basis of biopharmaceuticals. The venom glycan detection services we provide is suitable for different types of poisonous species or venom research. Such as fire-bellied toads, snakes, scorpions, frogs, venomous lizard, snails, spiders, bees, ants, cone snails, jellyfish, etc.

Introduction to Venom Proteomics Bioinformatics Services

Glycosylation is an important post-translational modification of proteins. According to the different connection modes of glycan chains and peptide chains, protein glycosylation can be divided into N-glycosylation and O-glycosylation. N-glycosylation is the connection of N-acetylglucosamine (Glc-NAc) at the reducing end of the glycan chain with the nitrogen atom of certain Asn side chain amido groups in the peptide chain. The Asn that can be connected to the glycan chain must be in the motif composed of Asn-X-Ser/Thr 3 residues, where X can be any amino acid residue except Pro. The structure of O-glycosylation is simpler than that of N-glycosylation. Generally, the glycan chain is shorter, but there are many more types than N-glycosylation. The glycosylation of peptide chains is mainly Ser and Thr, in addition to tyrosine, hydroxylysine and hydroxyproline, the connection site is the hydroxyl oxygen atom on the side chain of these residues.

Venom Glycan Detection Pipeline

Fig 1. The general pipeline of venom glycan detection.

Advantages of Creative Proteomics

• Advanced platform: The venom glycan spectrum detection adopts a mass spectrometry identification platform with ultra-high resolution, ultra-high sensitivity and fast scanning speed. Glycosylation analysis can be performed on peptides, proteins and antibody drugs, as well as the glycosylation sites, N-glycan /O-glycan types, and glycan composition of glycosylation sites in peptide or protein samples.
• Strict quality control: The experimental operation strictly follows the SOP for the detection of venom glycan, and multiple quality controls ensure that the experimental results are true and reliable.
• Personalized service: Provide personalized venom glycan detection service and customized biological information data analysis service according to the needs of customers.

Venom Glycan Detection Services

Creative Proteomics relies on years of glycan detection experience and uses different technical methods to provide a variety of glycan detection and analysis services for the majority of scientific researchers, such as venom N-glycan analysis and venom O-glycan analysis. Creative Proteomics provides a one-stop venom glycan detection and analysis service, which can be customized according to your specific needs. The one-stop glycan detection and analysis service we provide includes sample processing, sample detection, data analysis and project report output.

The venom glycan detection services we provide is suitable for different types of poisonous species or venom research. Such as fire-bellied toads, snakes, scorpions, frogs, venomous lizard, snails, spiders, bees, ants, cone snails, jellyfish, etc. Thank you for your attention to our venom glycan detection service! If you have any questions, please fill in the inquiry form and contact us immediately.