Venom Glycopeptides Analysis

Animal venom is a valuable source of biologically active peptides, which can be used as a potential source of medicine, even innovative. But animal venom contains not only some specific toxins, such as venom peptides, venom proteins, etc., but also small glycosylated peptides. Analysis of glycopeptides in the venom can link glycosylation information to the exact location of the protein (glycosylation site). Through liquid chromatography (LC) and mass spectrometry (MS) analysis, Creative Proteomics provides customers with analysis services of glycopeptide groups in different venoms.

Venom Glycopeptides Analysis

Glycosylation is the process of adding sugars to proteins under the control of enzymes. It is one of the important post-translational modifications of proteins and has important biological significance for the study of venoms. Glycosylation modification can affect the venom protein/peptide structure or determine the recognition and transduction process, and some relevant biomarkers are venom glycosylated peptides. Creative Proteomics uses LC-ionization-MS complementary technology to detect and characterize the largest range of glycopeptides in the venom. Our analysis services mainly include the following aspects:

• Monosaccharide diversity. Based on the molecular weight of glycosylated peptides, neutral loss and molecular weight of deglycosylated peptides, the presence of monosaccharides in the venom is identified.
• Glycoform diversity. Through the comparison of homologous sequences, different glycoforms of the same venom peptide can be identified.
• Nuclear Magnetic Resonance. The glycopeptide isolated from the venom was studied by nuclear magnetic resonance, and the amino acid side chains were observed and identified.

Technical Route

The analysis process uses a complementary method of LC-ionization-MS to detect and characterize the largest range of glycopeptides in the venom. In the LC-ionization experiment, the MS spectrum of all peptides in the target range is obtained by the technique of ionizing molecules directly from the liquid phase, and each spectrum is analyzed by looking for the typical neutral loss of monosaccharides. This method can quickly and effectively detect glycosylated peptides in the venom, and the combination of methods can maximize the characterization of unique glycosylated peptides.

Samples Delivery

• If you are providing a crude venom sample, please send the sample to us with dry ice.
• If you are providing an isolated protein/peptide sample, please tell us your extraction method and sample concentration.

Advantages

• The venom glycopeptide was characterized by a combination of various technical means.
• Enrichment of venom glycopeptides, low non-glycopeptide interference, high detection sensitivity.
• High specificity reveals glycopeptide group identification in complex venom.

As one of the leading companies in the field of venomomics. Creative Proteomics can rely on rich experience to cooperate with you, fully understand and overcome the research challenges you will face, and provide customized venom glycopeptide analysis services according to your needs. Contact us to discuss your project to find out what we can do for you.

Reference

• Michel D, et al. In-depth glyco-peptidomics approach reveals unexpected diversity of glycosylated peptides and atypical post-translational modifications in dendroaspis angusticeps snake venom. International Journal of Molecular Sciences, 2017, 18(11):2453.