Integrated Analysis of Transcriptomics and Lipidomics
Introduction
Transcriptome is a collection of complete mRNAs in cells under certain developmental stages or physiological conditions. Transcriptomics is the study of gene transcription in cells and the regulation of transcription at the overall level. Lipidome is the collection of all lipids in cells under specific developmental stages or physiological conditions. Lipidomics is an important branch of metabolomics, which is the analysis and identification of lipids in organisms, tissues or cells at the overall level, and the molecules that interact with it.
The integrated analysis of venom transcriptomics and lipidomics refers to the normalization and statistical analysis of batch data from the venom transcriptome and lipidome to establish the data relationship between venom mRNA and lipid molecules. Combining functional analysis, enrichment of metabolic pathways, molecular interaction and other biological function analysis, systematically and comprehensively analyze the functions and regulation mechanisms of venom, and finally realize a comprehensive understanding of the general trends and directions of biological changes. The integrated analysis of venom transcriptomics and lipidomics of venom or toxic species can analyze the biological functions of venom, such as synthesis mechanism, pathogenic mechanism, venom composition, and evolution, and provide a data basis for subsequent experimental research. At the same time, it also provides new ideas for the design and treatment of new venom-related drugs.
What Creative Proteomics Offers
The venom transcriptome sequencing service provided by Creative Proteomics is based on the Illumina high-throughput sequencing platform, which can detect the overall transcription level of any species or tissue at the single nucleotide level. In addition, it also provides lipidomics analysis services based on advanced tandem mass spectrometers and analysis software. With its rich experience in bioinformatics analysis, Creative Proteomics provides customers with customized venom transcriptomics and lipidomics integrated analysis services. From experimental design, sample sequencing, to multi-omics integrated data analysis, we provide you with high-quality one-stop venom transcriptomics and lipidomics integrated analysis services to meet a variety of research needs.
Method Flow
Fig 1. Transcriptomics and lipidomics integrated analysis pipeline - Creative Proteomics
Integrate Analysis Content
Venom transcriptomics and lipidomics joint analysis content:
- Venom transcriptome and lipidome multivariate statistical joint analysis: O2PLS association analysis, OPLS related variables screening.
- WGCNA analysis (recommended total biological replicates ≥ 15).
- Joint analysis of metabolic pathways: Visual analysis, Path comparison analysis, KEGG enrichment analysis.
- Transcriptome and lipidome correlation analysis: correlation coefficient matrix heat map, correlation analysis hierarchical clustering heat map, correlation coefficient regulation network analysis.
- Comparative analysis of PCA between venom transcriptome and lipidome group (limited to ≥3 groups)
Technical Advantages
Through the integrated analysis of venom transcriptomics and lipidomics, the following problems can be solved:
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Through the analysis of the expression levels of mRNA and lipids at different levels, the full spectrum analysis of venom gene expression and lipids can be realized.
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At the same time, it realizes the study of biological problems from the two directions of mRNA expression and lipid synthesis, and explores the regulation of specific gene expression on venom lipid synthesis.
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Analyze lipid data and mine lipid information related to venom. Then use correlation analysis to find important genes related to lipid changes. Through KEGG pathway analysis and correlation analysis of these genes, we can find important lipid metabolism enzymes that regulate key lipid changes, and finally clarify the relevant mechanism of venom synthesis.
Project Report
In the result report, Creative Proteomics will provide you with a detailed technical report, including:
- Experimental procedure (such as NGS and LC-MS/MS).
- Related experimental parameters.
- The raw sequencing data, including transcriptomics data and lipidomics data.
- Software and parameter settings used in the analysis process.
- Data analysis result pictures and tables.
How to Order?
Creative Proteomics provides customers with professional integrated analysis of venom transcriptomics and lipidomics data analysis services, aiming to help you understand the venom or poisonous species from the mRNA and lipid level. In addition, we also provide other different types of venom multi-omics joint analysis services. If you have any questions about our services, please feel free to contact us. Look forward to working with you！
For research use only. Not intended for any clinical use.
