Venom-Based Bioinsecticides Development

Although the widespread use of chemical pesticides provides solutions for the prevention and control of pests, it has brought hard-to-repair harm to our living environment. Therefore, we urgently need highly specific and environmentally friendly alternative products. Venomous animals such as spiders and ants contain many insecticidal toxins in their venom, which are not only a magic weapon for them as predators, but also have great potential to develop into biological insecticides.

Creative Proteomics provides services for the development of biological insecticides based on venom compounds discovery, and help customers obtain highly biologically active insecticidal toxins for pest control applications.

Our Services

We mainly provide research services to identify new insecticidal toxins in venom, and discover that toxins make it a potential source of compounds for the development of biological insecticides.

Identification of venom insecticidal toxins

• Bioassay-guided separation and purification of venom toxins. We collect the venom, conduct insect toxicity determination, separate the insecticidal peptides by RP-HPLC, and check the purity by LC/MS analysis.

Characterization of venom insecticidal toxins

• Characterization of venom insecticidal peptides. We use MS/MS for sequencing analysis, and manually interpret the obtained MS/MS spectra; use an automatic protein sequencer for Edman sequencing analysis, collect each separated fragment separately, and determine the structure of each fragment through MS/MS analysis.
• Characterization of insecticidal peptides derivatization. We separate and collecte the modified peptides by RP-HPLC, and the structure of the modified fragments will be confirmed by MS/MS analysis.

Application Strategies

We improve the toxicity of venom insecticidal toxin molecules by using carrier molecules or in combination with insect pathogens. Taking into account the specific needs of customers, such as insecticidal efficacy, specific selectivity, cost and other conditions, we select the most suitable venom toxin molecule for the development of biological insecticides.

One strategy is to transport venom insecticides to target insects by using different insect pathogens, such as viruses, entomopathogenic fungi and bacteria. This combined application can increase the insecticidal speed and insecticidal activity.

Another strategy is to transport venom insecticides to insects through carrier molecules, such as some water-soluble protein carriers, through conformational changes from pores or endocytosis to allow the toxins to enter the insect's blood and intestines.

Our Goal

We have unparalleled advantages in the exploration and characterization of venom. Based on the obvious advantages of venom insecticidal peptides, we are committed to satisfying the development needs of customers for new biological insecticides, and discovering new insecticidal peptides to the greatest extent possible to promote the development of biological pesticides. We strive to achieve the following goals:

• High efficiency
• High specificity
• High bioavailability
• High stability
• Low cost

Creative Proteomics has experienced venom researchers, the most advanced infrastructure, and is committed to providing the highest level of venom insecticidal peptide discovery and modification services to speed up the research process of your new pesticides. For more services, please contact us.

Reference

• Saez N J and Herzig V. Versatile spider venom peptides and their medical and agricultural applications. Toxicon, 2018.