De Novo Antibody Sequencing Service
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In the development of antibody drugs, access to the primary structural information of the antibody drug is fundamental and a prerequisite. Creative Proteomics' de novo antibody sequencing service enable accurate interpretation of the amino acid sequence of antibodies and play an important role in accelerating the discovery, development and study of antibodies.
What is De Novo Antibody Sequencing?
The process of generating the original gene sequence of a particular organism for the first time is known as de novo sequencing. De novo sequencing is a process for determining the amino acid sequence of the variable region of an antibody without knowledge of the antibody gene sequence. The technique plays an important role in antibody drug discovery and development. It is able to characterize the post-translational modifications and structural features of antibodies. This information is critical for understanding the stability, binding affinity and immunogenicity of the antibody, which are key factors in therapeutic antibody development.
Key Benefits of De Novo Sequencing
- Amino acid sequences can be obtained from their tandem mass spectra (MS / MS) without the help of a sequence database.
- Generate accurate reference sequences even for complex genomes.
- 100% sequence coverage.
- Identification of structural variants and complex rearrangements such as deletions, inversions or translocations.
- Elucidation of highly similar or duplicated regions to facilitate accurate de novo assembly.
One Stop De Novo Antibody Sequencing Services at Creative Proteomics
De novo antibody sequencing involves multiple steps including sample preparation, peptide fragmentation, mass spectrometry, and data analysis. Creative Proteomics combines our expertise with our platform technology to provide one-stop solutions. From monoclonal antibodies, polyclonal antibodies to antibody coupled drugs, our comprehensive services can definitely save your time and money.
- Peptide fragment digestion
Depending on the type of antibody sample, our experienced scientists select the appropriate protease enzyme to cut the peptide chain at specific amino acid residues to produce a mixture of small peptide fragments for subsequent analysis.
The fragmented peptides are then analyzed using mass spectrometry. In this technique, the peptides are ionized and accelerated through an electric field. As they move through the instrument, they are separated based on their mass-to-charge ratio. The resulting mass spectra provide information about the mass of the peptides.
The mass spectra obtained from mass spectrometry are then analyzed to determine the amino acid sequence of the antibody. This involves comparing the measured masses of the peptide fragments with the theoretical masses of all possible peptide sequences. Various bioinformatics tools and algorithms are used to identify the most likely sequence based on the mass spectra data.
- Confirmation and validation
Once a candidate sequence is identified, it needs to be confirmed and validated. This can be done using techniques like tandem mass spectrometry, where the selected peptide is fragmented again to provide additional information. Other methods like DNA sequencing or antibody gene synthesis can also be used to confirm the identified sequence.
Advantages of Our De Novo Antibody Sequencing Service
- Experienced team: Our team consists of highly skilled scientists and technicians who have years of experience in the field of genomics and sequencing. They possess the expertise to handle complex de novo sequencing projects and provide valuable insights and analyses.
- State-of-the-art technology: We utilize the latest advanced sequencing technologies, such as Next Generation Sequencing (NGS), to provide high-quality de novo sequencing. This ensures accurate and reliable results.
- Data analysis and interpretation: We not only provide raw sequencing data but also offer comprehensive data analysis and interpretation. Our bioinformatics experts employ advanced algorithms and software tools to unravel meaningful information from the generated sequencing data.
At Creative Proteomics, we specialize in antibody drug development and have a team of experienced scientists and experts in genetic analysis. Contact us to learn more about our De Novo antibody sequencing service for antibody drugs. We will be happy to assist you.
