Sedimentation Velocity Analytical Ultra Centrifugation (SV-AUC)

Sedimentation velocity analytical ultra centrifugation (SV-AUC) is the orthogonal technique of choice for the characterization and quantification of antibody therapeutic drug aggregates. Creative Proteomics offers powerful SV-AUC technology that provides customers with insight into the particle size distribution, purity, homogeneity and stability of their antibody drugs.

Introduction of Sedimentation Velocity Analytical Ultra Centrifugation (SV-AUC)

Sedimentation velocity analysis performed in the analytical ultracentrifuge (SV-AUC) provides a uniquely powerful first principal measure of the hydrodynamic size and shape of proteins under conditions that can come very close to the formulated drug product. This technique avoids the potential pitfalls associated with size exclusion chromatography (SEC) including on-column dilution, adsorption or disruption of species by a stationary phase, and the need to use high ionic strength mobile phases to screen unwanted electrostatic interactions. Furthermore, not only does SV-AUC provide a quantitative size distribution analysis, but it also provides information about macromolecular conformation. For these reasons, use of SV-AUC for analysis of therapeutic antibodies has become widespread throughout the biopharmaceutical industry and is one of the most common orthogonal techniques to SEC for measuring aggregate and fragment levels.

Application of SV-AUC in Antibody Drug Characterization

SV-AUC involves subjecting a sample containing the antibody drug to high centrifugal forces, causing the particles in the sample to sediment and separate based on their size and shape. As the sample is centrifuged, a laser beam is used to monitor the sedimentation of particles in real-time, allowing for the measurement of their sedimentation coefficient. By analyzing the sedimentation coefficient, we can help determine the following quality attributes of an antibody drug:

• Determination of the size and shape of antibody drug molecules.
• Analysis of aggregates in antibody drugs.
• Analyzes the unfolding or denaturation of an antibody based on the sedimentation coefficient, providing information about the stability of the antibody.
• Characterization of antibody drug conjugates binding efficiency.
• Antigen-antibody interaction detection.
• Batch consistency assay.

Advantages of SV-AUC

• Detection in the natural state of the sample, avoiding the interaction of the sample with the mobile phase and the column during SEC detection.
• Simple method, only need to adjust the rotational speed, can detect the molecular weight range of 102-108Da samples.
• Sample recovery.
• A label-free technique.

Our Capabilities

• With our efficient SV-AUC analysis tools, we offer comprehensive solutions for antibody drug characterization.
• Customized services to meet all client-specific demands.
• Comprehensive analysis reports provide in-depth information on antibody size, purity, aggregation and conformational stability.

Creative Proteomics offers a comprehensive SV-AUC-based characterization solution that enables researchers and developers to gain insights into their antibody drug candidates. Contact us to learn more about our service and we will be happy to serve you.