Submit Your Request Now

Isopentenyl adenosine Analysis Service

Isopentenyl adenosine is a member of the cytokinin family of plant hormones, which promote cell deivision by means of local and long distance signalling. Isopentenyl adenosine is abbreviated as iPA and the chemical molecular formula is C15H21N5O4.

Scientists at Creative Proteomics utilize a highly quantitative method with high-performance liquid chromatography (HPLC) for the determination of Isopentenyl adenosine levels in various samples, including Plant, Tissue and more. High-Performance Liquid Chromatography (HPLC) with UV detection is used for the determination of Isopentenyl adenosine (254 nm) levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of Isopentenyl adenosine measurement, which enables us to analyze of Isopentenyl adenosine levels in vitro and in vivo.

Isopentenyl adenosine is a naturally occurring nucleoside with an isopentenyl moiety derived from the mevalonate pathway. Isopentenyl adenosine and its derivatives can be found next to the anticodon, which is at position 37 in tRNA and is highly conserved from both the Bacteria and Eucarya domains. These modified nucleosides can improve the translational efficiency of tRNA as well as increase and decrease translational fidelity and lead to make the tRNA less codon context sensitive.The biosynthesis of Isopentenyl adenosine and its derivatives seems to be linked to iron metabolism and central metabolic pathways in bacteria. Isopentenyl adenosine has a well-established anti-tumor activity. Isopentenyl adenosine also has the ability to selectively expand and directly target human NK cells. Isopentenyl adenosine can stimulate the resting human NK cells and synergize IL-2, which can induce a robust activation and significant secretion of CCL5 and CCL3 at submicromolar doses. In addition, Isopentenyl adenosine can promotes NK cell proliferation and up-regulates the expression of specific NK cell-activating receptors, just as the expression of CD69 and CD107a. Isopentenyl adenosine may represent a novel therapeutic perspective as an immune-modulatory drug.

The Russian-Polish botanist M. Tswett is generally recognized as the first person to establish the principles of chromatography. In a paper he presented in 1906, Tswett described how he filled a glass tube with chalk powder (CaCO3) and, by allowing an ether solution of chlorophyll to flow through the chalk, separated the chlorophyll into layers of different colors. He called this technique “chromatography”. Fundamentally, chromatography is a technique used to separate the components contained in a sample. High Performance Liquid Chromatography (HPLC) is a method able to separate non-volatile, thermally unstable, and polar components separate or in a mixture. HPLC is a type of chromatography that, because of its wide application range and quantitative accuracy, is regarded as an indispensable analytical technique, particularly in the field of organic chemistry. It is also widely used as a preparation technique for the isolation and purification of target components contained in mixtures.

Isopentenyl adenosine Analysis Service at Creative Proteomics supports your research in Isopentenyl adenosine Analysis. HPLC Based Analysis Service Platform enable us at Creative Proteomics offers you a state-of-the-art Analysis Service.

Sample Type

Plant, Tissue and more

Method

High-Performance Liquid Chromatography (HPLC) with UV detection is used for the determination of Isopentenyl adenosine (254 nm) levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of Isopentenyl adenosine measurement, which enables us to analyze of Isopentenyl adenosine levels in vitro and in vivo.

Send us your samples, you will get all information that you need!

Metabolomics Sample Submission Guidelines

Download our Metabolomics Sample Preparation Guide for essential instructions on proper sample collection, storage, and transport for optimal experimental results. The guide covers various sample types, including tissues, serum, urine, and cells, along with quantity requirements for untargeted and targeted metabolomics.

Download

Metabolomics Sample Submission Guidelines

* For Research Use Only. Not for use in diagnostic procedures.

Our customer service representatives are available 24 hours a day, 7 days a week. Inquiry

From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

Online Inquiry

Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to for inquiries.

Great Minds Choose Creative Proteomics