Redox Proteomics Service

Creative Proteomics provides professional Redox Proteomics research services, focusing on the analysis of the dynamic changes of proteins in the REDOX state and their biological functions. Our services cover key modifications such as thiol oxidation (e.g. S-nitrosylation, S-glutathione), disulfide bond formation, and protein carbonylation, combined with high-resolution LC-MS/MS and specific enrichment techniques for precise identification and quantitative analysis of modified proteins. In addition, we provide bioinformatic analyses that provide insight into the role of redox modifications in cell signaling, metabolic regulation, aging, and diseases such as cancer and neurodegenerative diseases. With advanced experimental platforms and customized research protocols, we enable researchers to fully explore the mechanisms of redox regulation and advance biomedical research.

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What We Provide
Technology Platforms
Workflow
Advantages
Sample Requirements
Applications
Demo
FAQ
Publications

What Is Redox Proteomics?

Redox proteomics focuses on studying the modifications and dynamic changes of proteins during redox reactions^[1]. Among the most common redox modifications are the oxidation of thiol groups (-SH) in cysteine residues to form disulfide bonds (-S-S-) or mixed disulfide adducts (-SSG), as well as the oxidation of other amino acid residues, such as tyrosine and methionine. According to the different redox reactions, common redox modifications include S-nitrosylation, S-sulfination, S-glutathionylation, S-thiothiolate, intermolecular and intramolecular disulfide bonds, S-acylation. By systematically analyzing the modifications at these redox-sensitive sites, one can not only uncover the potential impact of oxidative stress on protein function but also further explore the fine-tuned regulatory mechanisms of intracellular redox homeostasis, which has important clinical significance in neurodegenerative diseases such as cancer and Alzheimer's disease^[2].

Redox Proteomics Service at Creative Proteomics

At Creative Proteomics, we have meticulously established and optimized our LC-MS/MS protocols to deliver a rapid, sensitive, and highly reliable site mapping service specifically designed for redox proteomics. Our advanced analytical platform integrates a high-performance C18 column with advanced instrumentation, including the Thermo Fisher Easy-nLC 1000 and the Thermo Fisher LTQ Orbitrap ETD. This cutting-edge setup allows us to achieve precise separation and high-resolution detection of redox modifications, enabling accurate identification and quantification of oxidative changes on proteins. Our comprehensive workflow covers every step—from robust sample preparation to detailed mass spectrometric analysis—ensuring that even subtle redox alterations are captured reliably.

Redox proteomics Research Platforms

Easy-nLC 1000 nanoliter liquid chromatography system

Easy-nLC 1000 (Figure from Thermo Scientific)

Thermo Fisher LTQ Orbitrap ETD mass spectrometer, combined with linear ion trap and Orbitrap technology, supports ETD fragmentation mode

Thermo Fisher LTQ Obitrap ETD (Figure from Thermo Scientific)

Workflow of Redox Proteomics Analysis

Workflow of redox proteomics analysis

Advantages of Our Redox Proteomics Service

Professional detection and analysis capability: Experienced research team, strict and matured techniques.
Breadth: We could skillfully deal with protein samples derived from a wide range of sources, encompassing animal and plant tissues, bacteria, blood, membrane proteins, etc.
High specificity and accuracy: Skillful quantification proteomics techniques, and PTMs enrichment methods.
High stability and reproducible: Obtain consistent and reproducible results for data analysis.
Advanced analytical platforms: Use of high-sensitivity mass spectrometry and advanced tools, ensuring precise and repeatable results.
End-to-End Support: Offer full-spectrum services from design to data interpretation, with continuous technical support throughout the research process.

Redox Service Sample Requirements

Sample Type	Sample Quantity
Tissue	animal tissue	200-500mg
Tissue	fresh plant	200-500mg
Cell	suspension cell	> 3x10⁷
	adherent cell	> 3x10⁷
	microorganism	> 50 mg or 3 × 10⁷ cells
Body fluid	Serum/plasma	1mL
Protein	Total protein > 3mg and concentration >1 μg/μL

Applications of Redox Proteomics Service

Biomarker discovery

Specific oxidation-modified proteins can be used as potential biomarkers for disease diagnosis and prognosis.

Drug action mechanism and target discovery

Evaluate how drugs affect cellular oxidative modification networks, reveal their mechanisms of action, and discover new therapeutic targets to aid drug development.

Mechanism of oxidative stress and disease^[2]

Systematically analyze how oxidative modification of proteins affects protein function, thereby revealing molecular mechanisms such as cancer, neurodegenerative diseases, and cardiovascular diseases.

Plant and agricultural research^[1]

Analysis of oxidative modification patterns of plants can optimize the study of crop stress resistance.

Demo Result of Redox Proteomics Service

The types of redox modifications identified and their proportions.

Venn chart^[3]

Volcano plot shows the significance and multiple changes of differentially modified proteins in redox proteomics data.

Volcano plot^[4]

Bubble maps howing the significance and enrichment of different proteins or pathways in redox proteomic analysis.

Bubble maps^[3]

PPI maps show the interactions between differentially modified proteins and help uncover key proteins or regulatory networks.

PPI Network^[3]

Motif enrichment heat maps of upstream and downstream amino acids at identified modification sites.

Heat map^[3]

FAQs of Redox Proteomics Service

How to ensure the stability of the REDOX state?

Reducing agents (such as DTT, TCEP) and alkylating agents (such as iodoacetamide) are used during sample processing to stabilize the REDOX state of cysteine, and experimental conditions are strictly controlled to avoid anthropogenic oxidation.

Can REDOX modifications be quantitatively analyzed?

Yes, mass spectrometry-based Label-free can be employed to accurately measure changes in REDOX modifications and provide quantitative results.

Which sample types are accepted by the service？

We accept a variety of sample types including cells, tissues, blood, serum, plasma, plant tissue and more. If you have other special sample types, please consult in advance.

Whether the sample needs pretreatment？

We recommend that customers provide fresh or frozen samples, which should avoid repeated freezing and thawing, and we will be responsible for subsequent protein extraction and pretreatment. If you have already extracted the protein, please communicate with us in advance about the sample handling method.

Learn about other Q&A.

Redox Proteomic Case Study

Article

Case Study: Redox Proteomic Analysis Reveals Microwave-Induced Oxidation Modifications of Myofibrillar Proteins from Silver Carp (Hypophthalmichthys molitrix)

Publications

Here are some publications in proteomics research from our clients:

Exploratory phosphoproteomics profiling of Aedes aegypti Malpighian tubules during blood meal processing reveals dramatic transition in function. 2022. https://doi.org/10.1371/journal.pone.0271248
Protein interactors of Spindle Pole Body (SPB) components and septal proteins in fungus Neurospora crassa: A mass spectrometry-based dataset. 2024. https://doi.org/10.1016/j.dib.2023.109980
Parkinson’s disease-associated LRRK2-G2019S mutant acts through regulation of SERCA activity to control ER stress in astrocytes. 2019. https://doi.org/10.1186/s40478-019-0716-4
Mechanistic and biomarker studies to demonstrate immune tolerance in multiple sclerosis. 2022. https://doi.org/10.3389/fimmu.2021.787498
Control of ribosomal protein synthesis by the Microprocessor complex. 2021. https://doi.org/10.1126/scisignal.abd2639

More Publications

References

Ramakrishnan, M., et al. "Redox status of the plant cell determines epigenetic modifications under abiotic stress conditions and during developmental processes." Journal of advanced research, (2022): 42, 99–116. https://doi.org/10.1016/j.jare.2022.04.007
Cadenas-Garrido P., et al. "Using Redox Proteomics to Gain New Insights into Neurodegenerative Disease and Protein Modification." Antioxidants (Basel, Switzerland), (2024): 13(1), 127. https://doi.org/10.3390/antiox13010127
Zhang, X., et al. "The Redox Proteome of Thiol Proteins in the Rice Blast Fungus Magnaporthe oryzae." Frontiers in microbiology, (2021): 12, 648894. https://doi.org/10.3389/fmicb.2021.648894
Montllor-Albalate, C., et al. Sod1 integrates oxygen availability to redox regulate NADPH production and the thiol redoxome. Proceedings of the National Academy of Sciences of the United States of America, (2022): 119(1), e2023328119. https://doi.org/10.1073/pnas.2023328119

Proteomics Sample Submission Guidelines

Ensure your samples are prepared and submitted correctly by downloading our comprehensive Proteomics Sample Submission Guidelines. This document provides detailed instructions and essential information to facilitate a smooth submission process. Click the link below to access the PDF and ensure your submission meets all necessary criteria.

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* For Research Use Only. Not for use in diagnostic procedures.

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From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

Online Inquiry

Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to for inquiries.