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Glycosphingolipid Glycans Analysis

Glycosphingolipids are ubiquitous in both cell membranes and serum. They either present in a free form or exist in a complex with some other proteins. The glycan head group moiety determines the biological function of glycosphingolipid. It is known to all that abnormal glycosphingolipid glycosylation is closely associated with many types of cancer, such as breast, lung cancer, and brain tumors. Glycosphingolipid glycans are also participating in many cellular processes, such as signaling and receptor functions, and cell differentiation. So it is of great value to analyze the structure of the head group of glycosphingolipid glycan in detail.

Comprehensive analysis of glycosphingolipid glycan requires the use of endoglycoceramidase, which has broad substrate specificity, and robust workflow for high-throughput analysis. Several steps are involved in glycosphingolipid glycans analysis:

Extraction and purification of glycosphingolipids from cell membranes or serum;
Release glycosphingolipid glycans with Endoglycoceramidase;
Permethylation of glycans
2AB labeling-UPLC-HILIC-FLD based analysis;
Permethylated glycans were analyzed by MALDI-TOF MS
Additionally, confirmation of those glycan structures by MS/MS fragmentation can be done if required.

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As one of the leading companies in providing omics analysis service, Creative Proteomics provides reliable glycomics analysis service for our customers. Contact us for more detailed information!

Proteomics Sample Submission Guidelines

Ensure your samples are prepared and submitted correctly by downloading our comprehensive Proteomics Sample Submission Guidelines. This document provides detailed instructions and essential information to facilitate a smooth submission process. Click the link below to access the PDF and ensure your submission meets all necessary criteria.

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* For Research Use Only. Not for use in diagnostic procedures.

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From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

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