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Lipid CLASS Profiles Service

Neutral lipids are the triglycerides which consist of three fatty acids attached to a glycerol molecule. Cholesterol is also a neutral lipid. Neutral lipids such as cholesterol esters and triglycerides are insoluble in water and essentially lack detergent properties. Phospholipids are a class of lipids that are a major component of all cell membranes. Phospholipids belong to the lipid family of biological polymers. A phospholipid is composed of two fatty acids, a glycerol unit, a phosphate group and a polar molecule. There are no simple methods available for analysis of phospholipids since the close range of polarity between different phospholipid species makes detection difficult.

Scientist at Creative Proteomics ultilize GC methods to Profile Neutral or Phospho-Lipids. Lipids are extracted from plasma, tissue or cells using a modified Folch method, and analyzed as either total lipid content, or separated on a TLC plate for analysis of each individual lipid class. Lipids are hydrolyzed prior to GC analysis. GC Analysis provides quantitation of lipids in each class by fatty acid chain length, but does not provide individual molecular structures. Researchers can choose how many total lipid classes to analyze, and should consult the Core Director for pricing based on the number of lipid classes requested. This unique method covers more than 1,100 molecular species across 16 lipid classes, including principle phospholipid, sphingolipid and neutral lipid classes.

Technology Platform:

Gas Chromatography

Sample Type:

Plasma, Tissue, Cells

Minimum sample quantity

Normal Volume: 200 uL plasma, 20 mg tissue, 1e7 cells
Minimal Volume: 50uL, 5 mg tissue, 6 e6 cells

Lipid Classes could monitored in This Service
Total Lipids TL-FA	Phospholipids PL-FA COMP	Neutral Lipids FA CompMG
14:0	14:0	14:0
14:1	14:1	14:1
16:0	16:0	16:0
16:1	16:1	16:1
18:0	18:0	18:0
18:1 (n-7)	18:1 (n-7)	18:1 (n-7)
18:1 (n-9)	18:1 (n-9)	18:1 (n-9)
18:2	18:2	18:2
18:3	18:3	18:3
20:0	20:0	20:0
20:1	20:1	20:1
20:2	20:2	20:2
20:3	20:3	20:3
20:4	20:4	20:4
20:5	20:5	20:5
22:0	22:0	22:0
22:1	22:1	22:1
22:4	22:4	22:4
22:5	22:5	22:5
22:6	22:6	22:6
24:0	24:0	24:0
24:1	24:1	24:1

Ordering Procedure:

Ordering Procedure

With integrated set of separation, characterization, identification and quantification systems featured with excellent robustness & reproducibility, high and ultra-sensitivity, Creative Proteomics provides reliable, rapid and cost-effective Lipidomics services.

Lipidomics Sample Submission Guidelines

Download our Lipidomics Sample Preparation Guide for essential instructions on proper sample collection, storage, and transport for optimal experimental results. The guide covers various sample types, including tissues, serum, urine, and cells, along with quantity requirements for untargeted and targeted lipidomics.

Download

Metabolomics Sample Submission Guidelines

* For Research Use Only. Not for use in diagnostic procedures.

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From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

Online Inquiry

Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to for inquiries.

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