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SILAC-based CoIP-MS Service

Co-immunoprecipitation (CoIP) is a classic method to study protein interactions based on the principle that antibodies specifically recognize specific antigens. CoIP is often used to examine the interactions between proteins under physiological conditions and can be used to identify and discover new protein interactions by combining with mass spectrometry (MS).

Our SILAC-based CoIP-MS Service

Creative Proteomics is capable of performing CoIP experiments after nonradioactive isotopic labeling of proteins using the SILAC assay. Then, immune complexes can be isolated and detected utilizing LC-MS/MS based on the specificities between the antigen and the antibody. More specifically, LC-MS/MS statistically detects the differences between proteins of the experimental group and the control group to determine protein interaction and protein networks. This means of detection greatly reduces false positive rates for protein interaction. Hence, the SILAC technology combined with CoIP-MS technology can be used for high-throughput quantitative analysis of protein interaction and networks for various applications.

The combined use of CoIP-MS technology and SILAC assay prevent systematic experimental error and support extensive quantitative information with high accuracy. Creative Proteomics uses Thermo Fisher's Q Exactive HF Mass Spectrometry platform, Orbitrap Fusion Mass Spectrometry platform, and Orbitrap Fusion Lumos Mass Spectrometry platform combined with Nano-LC to launch a SILAC-based CoIP-MS service package for protein interaction analysis. By using this service, customers will only need to send in the sample of investigation, inform objectives of experiments and leave the rest to our team, including but not limited to cell culture, cell labeling, protein extraction, antibody IP, efficiency detection, protease digestion, peptide segment separation, mass spectrometry analysis, mass spectrometry raw data analysis, and bioinformatics analysis.

Workflow of SILAC-based CoIP-MS

Features and Advantages of SILAC-based CoIP-MS

- Protein structure and post-translational modifications are preserved as much as possible
- Protein interactions are carried out in the unmodified confirmation and avoid human factor influences
- Interacting protein complexes can be isolated in unmodified confirmations
- High throughput rapid and comprehensive analysis of all interacting proteins
- Non-specific binding proteins can be identified in comparison with controls at low false positive rates
- Relative abundance of individual interacting proteins can be compared and analyzed

Sample requirements

Sample type (Dry ice transportation)	Sample requirements
cell	>5×10⁶
Protein extracts	Total protein > 200 μg, concentration >1 μg/μL

Report

1. Experimental procedures
2. Relevant mass spectrometry parameters
3. Details of identified proteins
4. Mass spectrogram
5. Raw data
6. Bioinformatics analysis

Proteomics Sample Submission Guidelines

Ensure your samples are prepared and submitted correctly by downloading our comprehensive Proteomics Sample Submission Guidelines. This document provides detailed instructions and essential information to facilitate a smooth submission process. Click the link below to access the PDF and ensure your submission meets all necessary criteria.

Download

* For Research Use Only. Not for use in diagnostic procedures.

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From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

Online Inquiry

Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to for inquiries.

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