Gibberellin is abbreviated as GAs and is a kind of plant hormones, which can regulate growth and have effect on various developmental processes, such as stem elongation, germination, dormancy, flowering, sex expression, enzyme induction, and leaf and fruit senescence and so on. Gibberellin is first discovered by a Japanese scientist named Eiichi Kurosawa in 1926 when he was studying bakanae, which is a disease that infects the rice plant. Gibberellin is then isolated by Teijiro Yabuta and Sumuki form fungal strains, named as Gibberella fujikuroi, which is provided by Kurosawa for the first time in 1935 and Yabuta named the isolated chemical compound as Gibberellin.
Scientists at Creative Proteomics utilize a highly quantitative method with high-performance liquid chromatography (HPLC) for the determination of Gibberellin levels in various samples, including Plant, Tissue and more. High-Performance Liquid Chromatography (HPLC) with UV detection is used for the determination of Gibberellin (254 nm) levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of Gibberellin measurement, which enables us to analyze of Gibberellin levels in vitro and in vivo.
Gibberellins are reported that participated in the biological nature process to breaking dormancy and other aspects of germination. Gibberellins are believed to involved in the starch hydrolysis signal pathway by inducing the biosynthesis of the enzyme α-amylase, which is an enzyme that catalyses the hydrolysis of starch into sugars in the aleurone cells. It has demonstrated that Gibberellins are produced in the scutellum and then are diffused to the aleurone cells, where Gibberellins can stimulate the releasing of α-amylase in the model for Gibberellin-induced production of α-amylase. Gibberellins are believed can induce higher levels of the transcription of the gene coding for the α-amylase enzyme, and then stimulate the synthesis of α-amylase.
The Russian-Polish botanist M. Tswett is generally recognized as the first person to establish the principles of chromatography. In a paper he presented in 1906, Tswett described how he filled a glass tube with chalk powder (CaCO3) and, by allowing an ether solution of chlorophyll to flow through the chalk, separated the chlorophyll into layers of different colors. He called this technique “chromatography”. Fundamentally, chromatography is a technique used to separate the components contained in a sample. High Performance Liquid Chromatography (HPLC) is a method able to separate non-volatile, thermally unstable, and polar components separate or in a mixture. HPLC is a type of chromatography that, because of its wide application range and quantitative accuracy, is regarded as an indispensable analytical technique, particularly in the field of organic chemistry. It is also widely used as a preparation technique for the isolation and purification of target components contained in mixtures.
Gibberellin Analysis Service at Creative Proteomics supports your research in Gibberellin Analysis. HPLC Based Analysis Service Platform enable us at Creative Proteomics offers you a state-of-the-art Analysis Service.
Sample Type
Various Sample Type
Method
High-Performance Liquid Chromatography (HPLC) with UV detection is used for the determination of Gibberellin (254 nm) levels in a lot of biological samples. This Methodology provides accurate, reliable, and reproducible results of Gibberellin measurement, which enables us to analyze of Gibberellin levels in vitro and in vivo.
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