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Propionylation Analysis Service

Gene expression in eukaryotes is tightly regulated, on the one hand by transcription factors; on the other hand, it is regulated at the epigenetic level. Histone modifications are an important part of epigenetics, and with the rapid development of high-resolution mass spectrometry, novel histone modifications can be identified. Among them, lysine propionylation modification is an important reversible post-translational modification of histone proteins discovered in recent years, which is widely involved in a series of biological processes and cellular metabolic pathways in prokaryotes and eukaryotes. In addition, propionylation modification has also been found to play an important role in the overall metabolic regulation network of bacteria and the activity regulation of metabolic enzymes, the cellular stress response of bacteria and mammals, the epigenetic regulation of eukaryotes and prokaryotes, as well as the regulation of photosynthesis.

Propionylation is the process of covalent binding propionyl groups (e.g., propionyl coenzyme A and other donors) to lysine residues in substrate proteins catalyzed by the action of acyltransferases (e.g., acetyltransferases).

Our Propionylation Proteomics Service

Creative proteomics offers the lysine propionylation modification service to identify propionylated proteins and their modification sites using high-resolution tandem mass spectrometry complementing the enrichment strategy.

Workflow

Customized Bioinformatics Services

Modified proteins and modification sites identification
Characterize identification results: peptide length, protein coverage distribution, unique peptide distribution
Modification site motif prediction
Protein GO functional classification, COG annotation, Pathway pathway annotation
Protein network interactions prediction (advanced analysis)
Protein domain prediction (advanced analysis)
Protein subcellular localization (advanced analysis)

Sample requirements

Fresh animal tissue: ≥600 mg
Fresh plant tissue: ≥6 g
Cell culture: ≥1×10⁷ cells/tube x 3 tubes
Fungi and bacteria: ≥600 mg
Serum, plasma: 450 μL × 4 tubes
Protein solution: total protein of 5-10 mg
Body fluid samples: urine of 15 mL × 4 tubes (centrifuge at 1000 x g for 5 minutes and discard sediment); or other body fluids (saliva, amniotic fluid, cell culture supernatant, etc.) > 15 mL

Advantages

High specificity and enrichment efficiency
Large-scale identification of enriched propionylated peptides with mass spectrometry of high resolution and high throughput
Combining commercially available quantitative techniques to analyze, compare, and correlate propionylation levels between samples quantitatively

Applications

Cellular stress response
Bacterial metabolic regulatory networks
Photosynthesis
Epigenetic regulation

Technology platform

Ion Chromatography
High-Performance Liquid Chromatography (HPLC)
Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (MALDI-MS)

Reference
1. Adam F Kebede, et al. Histone propionylation is a mark of active chromatin. Nature structural & molecular biology.(2017).

Proteomics Sample Submission Guidelines

Ensure your samples are prepared and submitted correctly by downloading our comprehensive Proteomics Sample Submission Guidelines. This document provides detailed instructions and essential information to facilitate a smooth submission process. Click the link below to access the PDF and ensure your submission meets all necessary criteria.

Download

* For Research Use Only. Not for use in diagnostic procedures.

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From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

Online Inquiry

Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to for inquiries.

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