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Steroids Analysis Service

A steroid is an organic compound with four rings arranged in a specific configuration, such as dietary lipid cholesterol, estradiol, testosterone and dexamethasone. Steroids function as important components of cell membranes and many of them are signaling molecules. The core structure of the steroid is composed of seventeen carbon atoms, bonded in four "fused" rings: three six-member cyclohexane rings and one five-member cyclopentane ring. Hundreds of steroids are found in plants, animals and fungi. All steroids are manufactured in cells from the sterols lanosterol (animals and fungi) or cycloartenol (plants). Lanosterol and cycloartenol are derived from the cyclization of the triterpene squalene.

Equipped with advanced LC/MS/MS sophisticated detection techniques, and a panel of experienced scientists and technicians. Steroids analysis service is able to quantification up to 38 steroids in biological matrices such as plasma, serum, tissue, cells, and cell culture media.

Sample preparation and extraction of steroids is by either liquid-liquid extraction (LLE) or supported liquid extraction (SLE) followed by an on-line 2D polishing extraction. Target steroid analytes are chromatographically separated on a reverse-phase (RP) column. All analytes and Internal Standards are measured by ESI ionization--pos and/or neg polarity (analyte dependent). Creative Proteomics provide customer-tailored service with rapid analysis procedures and easy to read report, to speed up your scientific research.

Technology Platform:

ESI Liquid Chromatography Mass Spectrometry (ESI-LC/MS)

Sample Type:

Plasma, serum, tissue, cells, and cell culture media

Sample Volume:

Normal Volume: 500 uL

Minimal Volume: 50uL

Steroids could monitored in This Service

∆4 Steroids could monitored in This Service
Aldosterone	Androsterone	Androstenedione
Corticosterone	Cortisol	Cortisone
11-deoxycortisol	11-deoxycorticosterone	Estradiol
Estrone	17-Hydroxyprogesterone	Progesterone
Testosterone	Dihydrotestosterone	11-Hydroxyandrostenedione
11-Hydroxyprogesterone	11-Hydroxytestosterone	11-Ketoandrostenedione
11-Ketoprogesterone	11-Ketotestosterone	16-Hydroxyprogesterone
18-Hydroxycortisol	18-Oxocortiso	21-Deoxycortisol

∆5 Steroids could monitored in This Service
Pregnenolone	17-hydroxypregnenolone	Allopregnenolone
5α-pregnane-3α,17α-diol-20-one (pdiol)	Dehydroepiandrosterone	5-Androstenediol

5α-Reduced could monitored in This Service
Dihydrotestosterone	Androsterone

Conjugated Steroids Sulfates could monitored in This Service
Pregnenolone sulfate	17-hydroxypregnenolone sulfate	Dehydroepiandrostenedione-sulfate
Androsterone sulfate	Etiocholane sulfate	Androst-5-ene-diol sulfate

Ordering Procedure:

Ordering Procedure

With integrated set of separation, characterization, identification and quantification systems featured with excellent robustness & reproducibility, high and ultra-sensitivity, Creative Proteomics provides reliable, rapid and cost-effective Lipidomics services.

Lipidomics Sample Submission Guidelines

Download our Lipidomics Sample Preparation Guide for essential instructions on proper sample collection, storage, and transport for optimal experimental results. The guide covers various sample types, including tissues, serum, urine, and cells, along with quantity requirements for untargeted and targeted lipidomics.

Download

Metabolomics Sample Submission Guidelines

* For Research Use Only. Not for use in diagnostic procedures.

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From Our Clients

"I recently used their proteomics service for a project analyzing protein interactions in yeast models. The team was very responsive and helped clarify the methodology they employed, which made me feel confident in the results. The data quality was solid, with clear identification of several key proteins involved in our study. Their thorough analysis enabled me to pinpoint specific interactions that I hadn't considered before, which significantly improved the direction of my research. I appreciate their professionalism and support throughout the process."

Sarah Thompson, University of California, Berkeley

"Our lab collaborated with them on a project studying cancer biomarkers. The proteomics analysis provided was detailed and focused, specifically highlighting the differential expression of proteins between healthy and tumor samples. Their clear explanations of the data helped my team understand the biological implications. I also appreciated their willingness to revise the reports based on our feedback, ensuring that we had everything we needed for our publication. This collaborative spirit was invaluable."

Emily Rodriguez, Stanford University

"Our lab worked with them on a project studying the effects of diet on gut microbiota using proteomics. They used a label-free quantification method to analyze proteins in fecal samples before and after dietary intervention. The results showed significant changes in protein expression linked to microbial activity. This was pivotal for our hypothesis about diet-microbiota interactions. The clarity of their data presentation made it easy for our team to integrate these findings into our ongoing research."

Dr. Lisa Wong, University of Toronto

"My experience with Creative Proteomics during the mass spectrometry analysis was excellent. We sent in human saliva and mouse brain tissue samples, which they expertly analyzed using both LC-MS and GC-MS techniques. The results were invaluable, revealing key metabolites in the saliva and identifying biomarkers linked to brain function in the brain tissue."

Dr. Emily Carter, Senior Research Scientist

"The overall service from Creative Proteomics was outstanding. They made the entire process seamless and efficient, allowing us to focus on our research. We worked with leaf and root samples from various Arabidopsis genotypes for targeted metabolomics analysis. Their thorough profiling of primary and secondary metabolites gave us important insights into how the plants respond metabolically to environmental stress."

Dr. Laura Henderson, Plant Physiologist

"We had a pleasant collaboration with Creative Proteomics on mass spectrometry analysis of lipids. They conducted a detailed analysis of lipid species, providing us with important insights into lipid metabolism and its relationship with metabolic syndrome disease states."

Dr. Sarah Mitchell, Research Scientist

Online Inquiry

Please submit a detailed description of your project. We will provide you with a customized project plan to meet your research requests. You can also send emails directly to for inquiries.

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