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What is Mass Spectrometry?
Mass spectrometry (MS) is an analytical technique used to measure the mass-to-charge ratio of ions. It produces a mass spectrum, a plot of intensity against m/z ratios, which allows for the identification and quantification of compounds in both pure samples and complex mixtures. MS is valuable in determining the elemental or isotopic composition of a sample, the masses of particles and molecules, and elucidating the chemical identity or structure of compounds. Mass spectrometry service companies typically utilize mass spectrometry technology to provide various biological analysis solutions for clients in pharmaceuticals, biotechnology, research institutions, and environmental monitoring. This helps clients achieve drug development, enhance research efficiency, optimize product quality, ensure environmental safety, and advance scientific research.
Applications of Mass Spectrometry
MS is a transformative analytical technique that provides high sensitivity and specificity for studying complex biological systems. At Creative Proteomics, MS is integral to various fields, including but not limitied proteomics, metabolomics, glycomics, and mass spectrometry imaging.
| Application | Details |
|---|---|
| Proteomics | Protein Identification: MS facilitates protein identification through techniques like peptide mass fingerprinting and de novo sequencing, matching mass spectra against databases to uncover known and novel proteins. |
| Protein Quantification: MS enables both relative and absolute quantification of proteins, employing methods such as isobaric tagging and label-free quantification to analyze protein expression levels across conditions. | |
| Post-Translational Modification Analysis: MS is essential for characterizing post-translational modifications (PTMs) like phosphorylation and glycosylation, allowing researchers to explore how these modifications affect protein function and dynamics. | |
| Protein Structure Analysis: MS aids in understanding protein structure through techniques like cross-linking and hydrogen-deuterium exchange, providing insights into protein folding and interactions. | |
| Protein Complex Analysis: MS allows for the analysis of protein complexes, revealing composition and interactions in their functional states via native mass spectrometry. | |
| Metabolomics | Untargeted metabolomics: utilizes MS to profile all metabolites in a sample, enabling the discovery of novel compounds and metabolic changes without prior knowledge of the metabolites. |
| Targeted Metabolomics: focuses on quantifying specific metabolites, using techniques like multiple reaction monitoring (MRM) for sensitive analysis of biochemical pathways. | |
| Mass Spectrometry Imaging | Mass spectrometry imaging (MSI) visualizes the spatial distribution of biomolecules in tissues, offering insights into the localization of metabolites and proteins, particularly in cancer research. |
| Glycomics | Glycomics involves studying glycans, and MS provides tools for analyzing glycan structures and their interactions with proteins, crucial for understanding biological processes like cell signaling and immunity. |
| Lipidomics | Lipidomics leverages MS to analyze lipid profiles, providing insights into lipid composition and metabolism, critical for understanding cellular signaling. |
| Other | Drug Development Environmental Analysis Clinical Research Food Safety and Quality Control |
Workflow of Mass Spectrometry
MS workflow involves sample preparation and quenching to maintain metabolite integrity, followed by separation using gas or liquid chromatography. The separated compounds are ionized, and the resulting ions are analyzed based on their mass-to-charge (m/z) ratios. Detection generates signals for data acquisition, producing a mass spectrum that allows for compound identification and quantification.
(Alseekh, S et al., Nature methods 2021)
LC-MS Analysis Service
Creative Proteomics specializes in providing top-tier proteomics and metabolomics services, with a focus on LC-MS analysis. This service is pivotal for the accurate identification and quantification of proteins and metabolites in complex samples, making it a preferred choice across various fields such as pharmaceuticals, biotechnology, and environmental analysis.
GC-MS Analysis Service
GC-MS Analysis Service combines Gas Chromatography (GC) and Mass Spectrometry to separate, identify, and quantify chemical compounds in various samples. This technique is highly effective for analyzing volatile and semi-volatile compounds, making it applicable in fields such as environmental testing, food and beverage analysis, forensic science, pharmaceutical quality control, and metabolomics. With its high sensitivity, specificity, and quantitative capabilities, GC-MS offers comprehensive analysis and detailed reporting
How is Mass Spectrometry performed?
Our Mass Spectrometry Facility features cutting-edge systems and a diverse array of ionization techniques for accurate analysis. With a comprehensive platform and advanced analysis software, we provide reliable data interpretation for various research applications in proteomics and beyond.
State-of-the-art Systems
- MALDI-TOF Mass Spectrometry
- Triple Quadrupole Mass Spectrometry
- Quadrupole-Trap Mass Spectrometry
- Hybrid Linear Ion Trap Orbitrap Mass Spectrometry
- Quadrupole-Orbitrap Mass Spectrometry
MS-DIAL，Mascot，Bruker – Compass Data Analysis，Agilent Technologies -MassHunter，MassLynx，Proteome Discoverer，Skyline，ProteoWizard
What Mass Spectrometry Services Do We Offer?
Mass Spectrometry Proteomics Service
Our proteomics analysis utilizes mass spectrometry services to identify proteins and quantify proteins within complex biological samples. By employing techniques such as liquid chromatography-tandem mass spectrometry (LC-MS/MS), we facilitate the detailed profiling of protein expression, post-translational modifications, protein interactions ,and more . This enables researchers to gain insights into cellular functions, disease mechanisms, and therapeutic targets, making it an essential tool in both basic and applied research.
Mass Spectrometry Metabolomics Service
In the realm of metabolomics, our mass spectrometry services allow for the comprehensive profiling of small molecules wuntarithin biological samples. Utilizing both targeted and geted approaches, we analyze metabolites to understand metabolic pathways and their implications in health and disease. This analysis provides valuable information for biomarker discovery, drug metabolism studies, and the assessment of metabolic diseases, thereby enhancing the understanding of biological processes at a metabolic level.
Mass Spectrometry Glycomics Service
Glycomics analysis at Creative Proteomics focuses on the characterization of glycans and glycoproteins using advanced mass spectrometry techniques. We assess glycosylation patterns, which are critical for protein function and stability, by employing methods such as MALDI-TOF and LC-MS. This service supports research in areas such as immunology, cancer biology, and drug development by elucidating the roles of carbohydrates in biological systems.
Mass Spectrometry Lipidomics Service
Our lipidomics services leverage mass spectrometry to analyze lipid species and their roles in cellular functions and disease states. By employing high-resolution mass spectrometry, we can profile a wide range of lipids, providing insights into lipid metabolism, signaling pathways, and the impact of dietary components on health. This analysis is crucial for understanding various conditions, including metabolic disorders and cardiovascular diseases.
Mass Spectrometry Bioinformatics Analysis Service
To complement our mass spectrometry services, we offer robust bioinformatics analysis that aids in the interpretation and integration of complex data sets. Our bioinformatics team utilizes advanced software tools to facilitate data processing, statistical analysis, and visualization, enabling researchers to draw meaningful conclusions from their mass spectrometry results. This service is essential for enhancing the reproducibility and reliability of experimental findings, ensuring that researchers can make informed decisions based on solid data.
Advantages of Our Mass Spectrometry Services?
Comprehensive Analytical Capabilities
Our mass spectrometry services encompass a broad spectrum of applications, including proteomics, metabolomics, glycomics, lipidomics, and bioinformatics analysis. This comprehensive approach allows us to provide in-depth insights into various biological systems, helping researchers to uncover complex interactions and mechanisms at play. Whether analyzing proteins, metabolites, or lipids, our team is equipped to handle diverse sample types and methodologies, ensuring that every aspect of your project is covered.
State-of-the-Art Technology
At Creative Proteomics, we utilize cutting-edge mass spectrometry platforms, including high-resolution instruments and advanced ionization techniques. Our facility is equipped with leading-edge technology such as the Q Exactive Hybrid Quadrupole-Orbitrap and the Sciex TripleTOF series, ensuring superior sensitivity and resolution for detecting analytes of interest.
Expertise and Experience
Our dedicated team of scientists possesses extensive knowledge in mass spectrometry and bioanalysis, enabling us to tailor services to meet the unique needs of each client. We collaborate with multiple research teams and showcase a rich output of results, reflecting our commitment to advancing scientific discovery. Our experience in developing and validating numerous methods across various applications translates into efficient project execution, allowing us to overcome challenges and deliver high-quality data that clients can trust.
Customized Solutions
Understanding that each research project presents unique challenges, we prioritize flexibility and communication with our clients. Our approach involves close collaboration, allowing us to tailor our mass spectrometry services to align with specific project goals and timelines. From method development to data interpretation, we seek client input at every stage to ensure that the outcomes meet expectations and drive research forward.
Data Integration and Bioinformatics
In today's multi-omics research landscape, integrating data from various sources is crucial for gaining comprehensive insights. Our bioinformatics services enhance our mass spectrometry analyses by providing sophisticated data integration capabilities. This includes the use of advanced statistical methods and machine learning approaches, allowing us to extract meaningful biological information from complex datasets and elucidate underlying biological pathways.
Preparation of Biological Samples for Mass Spectrometry
To prepare biological samples for MS, it is essential to follow systematic steps to ensure quality results. Begin by selecting the appropriate sample type (e.g., plasma, serum, urine, or tissue) based on the study's objectives. For proteomics, samples may require steps such asprotein precipitation, protein extraction, purification, and enzymatic digestion to generate suitable peptides. In metabolomics, sample processing includes immediately freezing biological samples to preserve metabolite stability, avoiding repeated freeze-thaw cycles after freezing, followed by thawing, centrifugation to separate cellular components, extraction using solvents such as methanol or acetonitrile, and purification and concentration of the extracted metabolites to remove contaminants before analysis. In glycomics, sample preparation typically includes processes such as sugar hydrolysis, enrichment, sugar release and labeling, and sugar derivatization.Finally, aliquot and label the samples appropriately for submission. For comprehensive instructions, we recommend downloading our Sample Submission Guidelines to facilitate a smooth submission process.
Sample Submission Guidelines
To ensure that your samples are prepared and submitted correctly, please download our guidelines.
Click the link to access the PDF and verify that your submission meets all necessary standards.
What is the principle of mass spectrometry?
Mass spectrometry (MS) operates on the principle of measuring the mass-to-charge ratio of ions. Samples are ionized, and the resulting ions are sorted based on their mass and charge in an electric and/or magnetic field, allowing for the identification and quantification of the molecules present.
What are matrix effects in mass spectrometry?
Matrix effects refer to the influence of other substances in a sample that can interfere with the ionization of the target analyte, potentially leading to inaccurate results. These effects can cause variations in signal intensity and can complicate data interpretation.
What samples can be submitted?
Various types of biological and non-biological samples can be submitted, including plasma, serum, urine, tissues, and environmental samples, depending on the specific mass spectrometry service offered.
What are the essential factors to consider when developing a bioanalytical method using mass spectrometry?
Key criteria include sensitivity, specificity, reproducibility, stability, and the method's ability to quantify the analyte over the desired concentration range. Additionally, regulatory compliance and method validation are essential.
What are the key differences between LC-MS/MS and LC-HRMS for quantitative analysis, and in what situations is each method most suitable?
LC-MS/MS is typically preferred for targeted quantification due to its high sensitivity and specificity, making it suitable for trace analysis. LC-HRMS, on the other hand, provides higher resolution and is advantageous for untargeted analyses, such as discovering novel compounds in complex matrices.
When is Mass Spectrometry Analysis used in Drug Development?
Mass spectrometry is used throughout drug development, including in pharmacokinetics, drug metabolism studies, biomarker discovery, and in the analysis of the chemical composition of formulations.
What is mass spectrometry vs. mass spectroscopy?
Mass spectrometry refers to the technique used to analyze the mass of ions, while mass spectroscopy is sometimes used interchangeably but can also refer to the field of study concerning the principles and applications of mass spectrometry.
What is HRMS in mass spectrometry?
High-Resolution Mass Spectrometry (HRMS) provides precise mass measurements, enabling the differentiation of ions that differ by very small mass increments. It is particularly useful for identifying complex mixtures and for structural elucidation.
What types of samples or compounds are typically analyzed using Mass spectrometry Services?
Mass spectrometry services typically analyze a wide range of samples, including small molecules, proteins, peptides, metabolites, lipids, and more from various biological and environmental matrices.
What types of analytical inquiries or challenges can Mass Spectrometry Services help resolve?
Mass spectrometry can address questions related to compound identification, quantification, structural characterization, metabolic pathways, and the analysis of complex mixtures, among others.
Can mass spectrometry be used for metabolomics?
Yes, mass spectrometry is a key technology in metabolomics, enabling the comprehensive analysis of metabolites within biological samples.
What is the difference between GC-MS and LC-MS metabolomics?
GC-MS is typically used for volatile and semi-volatile compounds, while LC-MS is suitable for non-volatile and polar compounds. Each method is selected based on the physicochemical properties of the metabolites of interest.
Can mass spectrometry be used in genomics?
While mass spectrometry is primarily used in proteomics and metabolomics, it can also play a role in genomics, particularly in the analysis of nucleotides and other small molecules related to genetic material.
How can I open the MS raw data?
MS raw data can be opened using specialized software compatible with the mass spectrometer used, such as Thermo's Xcalibur, Sciex's Analyst, or Agilent's MassHunter. These software packages allow users to visualize, analyze, and interpret the raw data files.
What is mass spectrometry proteomics service?
Mass spectrometry proteomics service involves the use of mass spectrometry to analyze and characterize proteins in a sample. This service enables the identification of protein structures, post-translational modifications, and quantification of proteins, facilitating insights into biological processes, disease mechanisms, and biomarker discovery.
What is mass spectrometry used for?
Mass spectrometry is used for various applications, including identifying chemical compounds, determining molecular weights, analyzing protein structures, quantifying metabolites, and detecting biomolecules in complex mixtures. It is widely applied in fields such as proteomics, metabolomics, environmental analysis, and pharmaceutical development.
Can you explain the techniques and methodologies used in Mass Spectrometry Analysis Services?
Mass spectrometry analysis services utilize a range of techniques tailored to specific samples and research goals. Liquid Chromatography-Mass Spectrometry (LC-MS) is a key method that combines liquid chromatography for the separation of both polar and non-polar compounds with mass spectrometry for accurate identification. Another important approach is Gas Chromatography-Mass Spectrometry (GC-MS), which pairs gas chromatography to separate volatile compounds with mass spectrometry for their quantification. Electrospray Ionization (ESI) generates charged droplets from liquid samples, while Matrix-Assisted Laser Desorption/Ionization (MALDI) employs a laser to vaporize and ionize molecules embedded in a solid matrix. Tandem Mass Spectrometry (MS/MS) enhances analysis by using multiple mass analyzers for in-depth structural information. Lastly, Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR MS) offers high resolution by measuring the frequencies of ions trapped in a magnetic field. Together, these methodologies enable robust and versatile analysis tailored to diverse research requirements.
What types of samples or compounds are typically analyzed using Mass spectrometry Services?
Mass spec services can analyze a wide range of samples, including proteins, peptides, small molecules, metabolites, oligonucleotides, lipids, and glycoproteins. Samples may come from biological fluids (such as blood, urine, and saliva), tissue extracts, and cultured cells.
How do you perform Mass Spectrometry services for small molecules, proteins, peptides, and oligonucleotides?
For small molecules, the sample is typically extracted and purified before analysis, often using techniques like solid-phase extraction. Proteins and peptides undergo enzymatic digestion (e.g., with trypsin), followed by LC-MS analysis. Oligonucleotides may be analyzed directly or after derivatization to enhance detection. Each type of sample may require specific pre-treatment steps to ensure optimal results.
How does a Mass Spectrometry Service Company ensure data accuracy, precision, and quality in their analyses?
A mass spectrometry service company ensures data accuracy and precision through rigorous quality control measures, including using calibrated instruments, standard operating procedures, and validated methods. Regular maintenance and validation of equipment, along with participation in inter-laboratory comparisons, help maintain high analytical standards.
How does engaging a Mass Spectrometry Service provider usually work, from sample submission to receiving results?
Engaging a mass spectrometry service provider typically involves several key steps, beginning with sample submission. Clients submit their samples along with relevant documentation detailing the specific analysis requirements and any pertinent project information. Once received, the service provider reviews the samples to ensure they meet the necessary criteria for analysis. The samples are then prepared and analyzed using the appropriate mass spectrometry techniques tailored to the project's needs. After the analysis is completed, the data is processed and interpreted by skilled technicians. Finally, clients receive a comprehensive report containing the results, including any relevant data visualizations and interpretations, allowing them to make informed decisions based on the findings. Throughout this process, effective communication between the service provider and the client ensures that expectations are met and any questions are addressed.
What is liquid chromatography-tandem mass spectrometry service?
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) service is an advanced analytical technique that combines the separation capabilities of liquid chromatography with the identification and quantification power of tandem mass spectrometry. This process begins with liquid chromatography, which separates complex mixtures of compounds, such as metabolites, drugs, or proteins, based on their chemical properties. The separated components are then introduced into the tandem mass spectrometer, where they are ionized and fragmented for highly sensitive and specific detection. Widely used in pharmaceutical development, clinical diagnostics, environmental monitoring, and food safety testing, LC-MS/MS is valued for its accuracy and ability to analyze multiple compounds simultaneously, making it an essential tool for researchers and industry professionals.
Unbiased insights into the multiplicity of the CYP46A1 brain effects in 5XFAD mice treated with low dose-efavirenz
Natalia Mast ∙ Makaya Butts ∙ Irina A. Pikuleva
Journal: Journalof Lipid Research
Year 2024
Polyamine metabolism impacts T cell dysfunction in the oral mucosa of people living with HIV
Mahalingam, S. S., Jayaraman, S., et al
Journal: Nature Communications
Year 2023
Trypanosoma cruzi DNA Polymerase β Is Phosphorylated In Vivo and In Vitro by Protein Kinase C (PKC) and Casein Kinase 2 (CK2)
Edio Maldonado, Diego A. Rojas, Fabiola Urbina, et al
Journal: Cells
Year 2022
Characterizing the proteome of bullous pemphigoid blister fluid utilizing tandem mass tag labeling coupled with LC–MS/MS
Solimani, F., Didona, D., et al.
Journal: Archives of Dermatological Research
Year 2022
Identification of the O-Glycan Epitope Targeted by the Anti-Human Carcinoma Monoclonal Antibody (mAb) NEO-201
Kwong Y. Tsang, Massimo Fantini, Anjum Zaki, et al
Journal: Cancers
Year 2022
Loss of G0/G1 switch gene 2 (G0S2) promotes disease progression and drug resistance in chronic myeloid leukaemia (CML) by disrupting glycerophospholipid metabolism
Gonzalez, M. A., Olivas, I. M., et al.
Journal: Clinical and Translational Medicine
Year 2022
