Protein Profiling Analysis Service
Protein profiling is the comprehensive detection of proteins in a sample, which is the key to comprehensively researching the proteome and constructing a protein map. As a company that has specialized in proteomics research for many years, Creative Proteomics can provide professional protein profiling analysis services to facilitate your protein research.
What is Protein Profiling Analysis
Protein profiling is a comprehensive analysis of proteins in biological samples, involving the systematic identification and characterization of the entire proteome in the sample. It aims to provide insights into the types, amounts, modifications, interactions and functions of proteins in a particular biological environment. MS-based proteomics identifies and characterizes proteins in samples by ionizing and fragmenting peptides in protein digests. It is possible to gain a deeper understanding of cellular processes, disease mechanisms, and biological systems through protein profiling. With technological innovation and the integration of multi-omics, it will continue to advance.
The Principle of Protein Profiling Analysis
There are many intact proteins of different molecular weights, solubilities and modifications in the samples. When using mass spectrometry to identify protein profiling, the proteins are digested into peptide fragments, which are then identified by shotgun proteomics. Shotgun proteomics is based on the principle that the proteins in the sample are first digested into peptides, which are then separated by HPLC. Next, the separated peptides are detected directly by primary mass spectrometry (MS1) and secondary mass spectrometry (MS2). MS1 measures the m/z and ion intensity of the peptide, and then the peptide is divided into different windows for MS2 detection. The peptide entering MS2 is fragmented, and then MS2 detects the ion fragments to obtain the peptide's spectral data. Compare the detected data with the appropriate databases, and the complete protein sequence can be obtained through analysis, thus realizing protein profiling analysis. Shotgun proteomics could acquire large amounts of data in a short period of time with its high-throughput assays, making it an excellent choice for protein profiling analysis.
Our Service
Accurate protein profiling is essential for the research into protein expression, modification, interaction, function and more. It helps advance a number of fields such as disease research, drug discovery and personalized medicine. Creative Proteomics provides considerate and high-quality protein profiling analysis services no matter what methodology it relies on. We are also able to perform protein identification and protein interaction analysis with the help of our in-house labs.
Fig. 1. Protein profiling analysis workflow
Applications of Protein Profiling Analysis
Protein profiling comprehensively detects proteins in samples and is used in a variety of fields such as disease research, drug discovery, biopathways, personalized medicine, and so on. It can be used to identify biomarkers and drug targets, evaluate drug efficacy, reveal signaling pathways, analyze proteins expression under different conditions or in different tissues, and deduce protein functions, etc.
Advantages of Protein Profiling Analysis
- It comprehensively identifies a large number of proteins with high coverage.
- It can effectively identify low abundance proteins.
- It can infer protein function.
Creative Proteomics provides considerate and high-quality proteomics analysis services. If you are interested in protein profiling analysis, please contact us for more details.
