Isoflavonoids Analysis Service

What are Isoflavonoids and Why Analyze Them?

Isoflavonoids form a class of flavonoids, which are secondary metabolites ubiquitously present in leguminous plants. The incredible health benefits attributed to these compounds across numerous medical and scientific journals have brought them to the limelight in recent years.

Intake of isoflavonoids has shown strong inverse correlations with various cardiovascular diseases, different types of cancer, especially breast and prostate cancer, as well as menopausal discomforts. Their antioxidant, anticancer, and estrogenic/anti-estrogenic properties make these compounds potential candidates for drug production. Furthermore, isoflavonoids play crucial roles in assisting plants in warding off diseases and assaults from pests.

The analysis of isoflavonoids hence provides a comprehensive understanding of these compounds from their structural properties to their potential therapeutic applications.

Isoflavonoids Analysis at Creative Proteomics

At Creative Proteomics, we are committed to unboxing the untapped potential of isoflavonoids. Our analytical services cater to the requirements of food safety, pharmacology, environmental studies, and much more. Our offerings include but are not limited to:

• Isoflavonoid Quantitative Analysis: This service involves accurate quantification of various isoflavonoids in different samples such as food, beverages, and other biological samples. It is performed using advanced analytical techniques like HPLC, UHPLC, GC-MS/MS, and LC-MS/MS.
• Isoflavonoid Profiling: Isoflavonoid profiling service allows for comprehensive analysis and identification of the various isoflavonoids present in your samples. This helps in understanding the detailed isoflavonoid composition and could be crucial in product development and quality control processes.
• Isoflavonoid Metabolic Pathway Analysis: We offer exploration of the metabolic pathways of isoflavonoids, which can help researchers better understand their pharmacokinetic properties and mechanism of action.
• Isoflavonoid Derivatives Analysis: This service can help to identify and quantify various isoflavonoid derivatives which may have different biological activities from the parent compounds.
• Isoflavonoid Bioavailability Studies: Understanding the bioavailability of isoflavonoids is essential for determining their therapeutic potential. We provide services to evaluate how well these substances can be absorbed and utilized by the body.
• Isoflavonoid Function and Activity Studies: We conduct in-depth research on isoflavonoid function and activities, aiding in understanding their potential benefits and risks, as well as exploring their therapeutic applications.
• Customized Isoflavonoid Research Services: We also offer tailor-made research services depending on your specific project needs in the isoflavonoid research field.

Isoflavonoids Analysis Techniques

Sample Preparation Techniques: Tailored extraction methods, including Solid-Phase Extraction (SPE) and Liquid-Liquid Extraction (LLE), are utilized for diverse sample types, optimizing recovery and purity.

Chromatographic Separation: Liquid Chromatography (LC), specifically High-Performance Liquid Chromatography (HPLC), is employed for effective chromatographic separation, enhancing mass spectrometric selectivity and ensuring accurate results.

Triple Quadrupole Mass Spectrometry: Utilizing the Agilent 6460 triple quadrupole mass spectrometer for targeted quantification, this technique ensures unparalleled sensitivity for accurate concentration determination.

High-Resolution Mass Spectrometry: Our use of High-Resolution Mass Spectrometry with the Thermo Scientific Orbitrap allows for precise identification and profiling of isoflavonoids, broadening the range of detectable compounds.

Quality Control Measures: Stringent quality control measures, including calibration standards, internal standards, and quality control samples, validate instrument performance and ensure the accuracy and precision of the analysis.

Data Analysis and Interpretation: Thorough data analysis, encompassing peak integration, compound identification, and concentration determination, is conducted using advanced software tools. The results are interpreted by our experts, providing clients with comprehensive reports for actionable insights.

Workflow for Plant Metabolomics Service

List of Isoflavonoids Analyzed (including but not limited to)

Sample Requirements for Isoflavonoids Assay

Deliverables of Isoflavonoids Analysis

Our commitment to transparency and customer satisfaction prompts us to ensure our clients stay well-informed throughout the project lifecycle. The deliverables from our Isoflavonoids Analysis encompass:

• Detailed experimental protocol and procedure
• Raw data and processed data
• Comprehensive analysis report
• Expert insights and recommendations based on the analysis