Neoflavonoids Analysis Service

What are Neoflavonoids and Why Analyze Them?

Neoflavonoids—unique and highly potent subclass of flavonoids—are among the essential components in nature that are offering pharmacological benefits. Primarily found in families, such as Fabaceae, they have captured the attention of researchers globally due to their potential as therapeutic agents. Neoflavonoids are known for having striking and diverse bioactivities that include antimicrobial, antioxidant, antitumor, and anti-inflammatory properties, among many others.

Unlike traditional flavonoids, Neoflavonoids are characterized by a structural rearrangement of the C ring in the flavonoid nucleus. This unique structural variation imparts them with diverse bioactive properties, making them efficacious in different health-related applications.

Neoflavonoids analysis involves the qualitative and quantitative investigation of different species of neoflavonoids in various specimens. Analyzing them can provide critical insights into optimally leveraging their potential benefits. Such an analysis contributes to a multitude of disciplines —from pharmacology and phytochemistry to food science and industrial applications.

For instance, it can aid in the development of novel therapeutic drugs or agents, culminate in the advancement of dietary supplements, help in monitoring and controlling their levels in the environment, and boost the quality control protocols of both pharmaceutical and natural product industries.

At Creative Proteomics, we provide reliable and accurate neoflavonoids analysis utilizing state-of-the-art analytical techniques and instruments.

Neoflavonoids Analysis at Creative Proteomics

Neoflavonoids Identification and Quantification

We provide accurate identification and quantification of various neoflavonoids in different samples such as plant extracts, biological fluids, and pharmaceutical formulations. This service can be beneficial in several aspects including, but not limited to, studying the impact of neoflavonoids on human health, determining the concentrations of these compounds in nutraceuticals, and exploring their influence on environmental health.

Comparative Analysis of Neoflavonoids

We conduct comparative analyses to understand the differences in concentration and presence of neoflavonoids in different plant varieties or samples prepared under varying conditions. Such analyses can offer insights into the effect of various factors such as environmental conditions, genetics, and post-harvest treatments on the production of neoflavonoids.

Metabolomic Profiling of Neoflavonoids

This service involves the comprehensive study of the metabolome, which constitutes all the metabolites, including the neoflavonoids, in a biological sample. It can offer significant insights into the functional state of a biological system.

Tracing the Metabolic Pathways of Neoflavonoids

We offer this service to explore the metabolic pathways of neoflavonoids in various organisms. It helps in understanding the biological roles of these compounds and their metabolites.

Neoflavonoids Analysis Techniques

Liquid Chromatography-Mass Spectrometry (LC-MS)

LC-MS is employed extensively because it can rapidly analyze a wide range of neoflavonoids with high sensitivity and selectivity. Creative Proteomics utilizes advanced LC-MS instruments for the analysis of neoflavonoids, including QTRAP LC-MS/MS and high-resolution LC-MS (Q Exactive).

• QTRAP LC-MS/MS: A hybrid triple quadrupole/linear ion trap mass spectrometer. It allows for simultaneous quantitative and qualitative analysis, achieving high-efficiency and high-sensitivity analyses.
• Q Exactive LC-MS: A high-resolution, accurate-mass (HRAM) system. It combines quadrupole precursor selection with high-resolution, accurate-mass (HRAM) Orbitrap detection, ideal for untargeted screening and quantitation of neoflavonoids.

Gas Chromatography-Mass Spectrometry (GC-MS)

Another technique often used in neoflavonoids analysis is GC-MS. Although it requires the derivatization of neoflavonoids, it provides good separation and quantitative results for volatile and thermally stable neoflavonoids.

Although the above mentioned are standard analytical techniques, specific neoflavonoids may need customized analytical methodologies. At Creative Proteomics, we offer method development services for challenging compounds or matrices.

Workflow for Plant Metabolomics Service

List of Neoflavonoids Analyzed (including but not limited to)

Sample Requirements for Neoflavonoids Assay

Deliverables of Neoflavonoids Analysis

• Details of sample preparation and analysis procedures
• List of identified Neoflavonoids with their retention times (Rt), mass-to-charge ratio (m/z), and relative quantification values
• Raw data files
• Interpretations and conclusions drawn from the analysis