Amino Acid Metabolic Flux Analysis

Amino Acid Metabolic Flux Analysis

Metabolic flux analysis (MFA) is a powerful tool for determining intracellular fluxes. Creative Proteomics is a professional contract research organization (CRO) company that leverages its MFA platform to meet the needs of our customers for metabolites research in many fields. We can provide solutions for MFA of a range of mature cellular and tissue samples based on ¹³C or ¹⁵N stable isotope labeled metabolic precursors, such as D-Glucose (U-¹³C₆), D-Glucose (1,2-¹³C₂), D-Glucose (1-¹³C), L-Glutamine (¹³C₅), L-Glutamine (amine-¹⁵N), L-Glutamine (1-¹³C), and L-Glutamine (5-¹³C) for central carbon metabolic pathways, fatty acid, nucleotide and amino acid metabolism.

Amino acid metabolism

Amino acids are widely involved in metabolism, form the basic components of peptides and proteins, and play a vital role in biological processes. Amino acid metabolism is receiving increasing attention in the field of cancer metabolism. For example, amino acid profiles have been used for cancer detection. The Warburg effect is being re-evaluated due to new findings regarding the importance of glutamine as an energy source for cancer cell proliferation. In addition, glycine has recently been reported as an important metabolite in human cancers, as it is also strongly associated with the rate of proliferation of cancer cells.

Amino acid metabolic flux analysis service at Creative Proteomics

Intensive studies of amino acids and their metabolism may lead to significant discoveries in many areas of research, such as bioscience, disease diagnosis, and drug discovery. As a systems biology approach, MFA is used to study the metabolic pathways of compounds in cells or tissues and the flux of metabolites through these pathways. MFA can be applied to the study of amino acid metabolism, involving glutamine metabolism, serine metabolism, tryptophan metabolism, and so on. Based on years of experience on MFA and advanced MS platform, we provide amino acid metabolic flux analysis services, of which the following steps are involved:

• Sample collection
• Quenching and extraction
• Pre-experiments to optimize target parameters
• Preparation of samples to be tested
• On-board testing
• Metabolic flux content calculation
• Comprehensive statistical analysis
• Metabolic flux result and interpretation, including variance analysis, cluster analysis, correlation analysis, ROC analysis, network analysis, and other custom analysis

Accepted samples

Cells, tissues, microbial organisms, culture media and fermentation broth

Advantage of our service

• Quantitatively characterize the entire metabolic process and its precise flux distribution as well as identify rigid nodes of metabolic pathways
• Cover multiple metabolic pathways to meet multiple research needs
• Experienced in large-scale projects
• Multiple quality control systems for more accurate results

Our amino acid metabolic flux analysis can help researchers and professionals to investigate the molecular mechanism of amino acid metabolism regulation and provide important clues for the diagnosis and treatment of amino acid metabolism-related diseases. If you have are interested in our service, please don't hesitate to contact us. We look forward to working on your next project.

Reference

1. Gu, Haiwei, et al. "Metabolomics method to comprehensively analyze amino acids in different domains." Analyst 140.8 (2015): 2726-2734.