Metabolite Identification, Profiling & Characterization

Metabolite Identification, Profiling & Characterization

The investigation of metabolites play important roles in supporting drug discovery. A diversity of technologies have been employed in the metabolite identification, profiling and characterization. Profiling of metabolite is used to measure the conversion of a compound of interest into its metabolites and intermediates in a biological sample as a result of test systems such as enzymes, hydrolysis, gene modifications and physiological pathways. It studies what effect this compound has on the full set of metabolites in a broader set of biological systems. Moreover, characteristics and structural elucidation of metabolites can help scientists to identify human-specific metabolites. Aiming to address critical issues around biotransformation and drug metabolism, Creative Proteomics focuses on answering those critical questions by using context-driven analytical techniques.

Application

• Understand the mechanisms of clearance
• Identify the presence of potential active or reactive metabolites
• Observe the unique metabolites in the compound of interest
• Study the molecule's biotransformation capabilities

Figure 1. The overview of the two-step metabolite identification framework. (Markus, H.; et al. 2012)

Our Services

Creative Proteomics has developed a novel metabolic flux analysis platform to provide metabolite identification, profiling & characterization service in a competitive fashion. We can offer a wide range of services to support all research and development activities.

Metabolite identification

• Our teams perform radiochemical detection and quantification of metabolites or with gas chromatography for analysis of different types of chemistry
• We can provide critical information on the formation of metabolites including both phase I and phase II metabolism, and comparison of drug metabolism routes in different species

Metabolite profiling & characterization

• Creative Proteomics offers a comprehensive metabolite profiling and structural elucidation service for the profiling, isolation, analysis, and identification of metabolites in biological matrices in support of discovery and safety studies
• We offer metabolite profiling & characterization studies carefully designed to meet regulatory expectations for investigational new drug applications. Our comprehensive profiling & characterization results can tell you:
• Number of metabolites detected in each species
• Proposed biotransformation process leading to their formation
• UV peaks for the parent and metabolites
• Proposed metabolite structure and fragment assignment

Metabolite study protocol

• Matrices Analyzed

Creative Proteomics supports metabolites identification in a number of different matrices including in vitro microsomal incubations, hepatocyte incubations, expressed enzyme incubations as well as in vivo samples

• Instruments

Liquid chromatography-mass spectrometry
HPLC/UPLC
QToF and Orbitrap
Ion mobility separation
On- and offline radio detection
Thin Layer Chromatography Metabolite isolation
NMR (through partner)

• Data Delivery

Summary document with chromatograms and ion spectra
Customized report without structural elucidation
Comprehensive report ranging from a simple table of proposed structures to provision of extracted ion chromatograms, MS and MS/MS spectra with interpretation of the data for each metabolite

Figure 2. An example of a metabolite profile. (Kopka, J.; et al. 2004)

Features of Our MFA Platform

• Developed based on the most updated knowledge of biology, bioinformatics and software development
• Widely applicable to a wide range of metabolic system
• Professional bioinformatics teams & personalized bioinformatics analysis services.
• Advanced instrument platform
• Integrated quantitative methodologies and comprehensive solutions for metabolomics

Based on high-performance quantitative techniques and advanced equipment, Creative Proteomics has constantly updating our metabolic flux analysis platform and is committed to offering professional, rapid and high-quality services of metabolite identification, profiling & characterization at competitive prices for global customers. Our personalized and comprehensive services can satisfy any innovative scientific study demands, please contact our specialists to discuss your specific needs. We are looking forward to cooperating with you!

References

1. Markus, H.; et al. Metabolite identification and molecular fingerprint prediction through machine learning. Bioinformatics. 2012. 18(15): 2333-2341.
2. Kopka, J.; et al.Metabolite profiling in plant biology: platforms and destinations. Genome Biology. 2004. 5(6): 109.