Fatty Acid Metabolic Flux Analysis

Fatty Acid Metabolic Flux Analysis

Fatty acid metabolism, as an important component of lipid metabolism, is important for the growth and development of normal cells. Studies have shown that it also plays an important role in the occurrence, development and metastasis of tumors. Creative Proteomics provides reliable, rapid and cost-effective metabolic flux analysis services based on highly stable, reproducible and sensitive separation, characterization, identification and quantitative analysis systems. We offer leading international services in metabolic flux analysis (MFA) of multiple pathways, including fatty acid MFA.

Fatty acid and fatty acid metabolism

Fatty acids are a class of compounds composed of carbon, hydrogen and oxygen, and are the major components of neutral fats, phospholipids and glycolipids. Under sufficient oxygen supply, fatty acid can be oxidized and decomposed into CO₂ and H₂O, releasing a large amount of energy, so it is one of the primary energy sources of the body. According to the length of carbon chains in the molecular structure of fatty acids, there are three types of fatty acids: short-chain fatty acids, medium-chain fatty acids and long-chain fatty acids. Fatty acid metabolism consists of various metabolic processes involving or closely related to fatty acids, mainly including energy-producing catabolic and anabolic processes. In catabolism, fatty acids are metabolized to produce energy, mainly in the form of adenosine triphosphate (ATP). The sources of fatty acids include food intake and internal synthesis, i.e. exogenous and endogenous fatty acids. The synthesis of fatty acids takes place in the cytoplasm, and the direct raw material is acetyl CoA, which consumes ATP and NADPH to first produce sixteen-carbon soft fatty acids, which are processed to produce various fatty acids in the human body.

Fig.1 Fatty acid uptake, synthesis, and metabolism pathways. (Nagarajan, Shilpa R., Lisa M. Butler, and Andrew J. Hoy. 2021)

Fatty acid metabolic flux analysis service at Creative Proteomics

Creative Proteomics is a national high-tech enterprise, whose business scope mainly revolves around metabolic logistics detection, engaging in metabolite analysis and structural analysis and other related technical services, providing customers with cost-effective and efficient technical services. We can provide solutions for MFA of a range of mature cellular and tissue samples based on ¹³C or ¹⁵N stable isotope labeled metabolic precursors, such as D-Glucose (U-¹³C₆), D-Glucose (1,2-¹³C₂), D-Glucose (1-¹³C), L-Glutamine (¹³C₅), L-Glutamine (amine-¹⁵N), L-Glutamine (1-¹³C), and L-Glutamine (5-¹³C). Based on years of experience on MFA and advanced MS platform, Creative Proteomic provides amino acid metabolic flux analysis services. Our services can help researchers and professionals to quantitatively characterize the entire fatty acid metabolic process and its precise flux distribution, and contribute to the identification of rigid nodes of the fatty acid metabolic pathway.

Specifically, our service includes the following lists:

• Experimental design
• Labeling experiments
• Isotopic labeling
• External rate determination
• Fatty acid metabolic flux estimation
• Comprehensive statistical analysis
• Metabolic flux result and interpretation, including variance analysis, cluster analysis, correlation analysis, ROC analysis, network analysis, and other custom analysis

Accepted samples

Cells, tissues, microbial organisms, culture media and fermentation broth

Instrument platform

Orbitrap LC-MS, UHPLC-QQQ-MS, and GC-Q-MS

Common diseases associated with fatty acids include cardiovascular disease, metabolic syndrome, diabetes, and liver disease. A growing number of studies have suggested that altered fatty acid metabolism is a hallmark and metabolic phenotype of cancer cells. In-depth study of fatty acid changes in the tumor microenvironment may provide a new marker or therapeutic target to improve the prognosis and treatment of cancer. If you have are interested in our service, please don't hesitate to contact us. We look forward to working on your next project.

Reference

1. Nagarajan, Shilpa R., Lisa M. Butler, and Andrew J. Hoy. "The diversity and breadth of cancer cell fatty acid metabolism." Cancer & Metabolism 9 (2021): 1-28.