Cell Surface Proteomics

Cell surface proteomics is a specialized branch that focuses on the comprehensive analysis of proteins present on the outer membrane of cells, providing valuable insights into cellular communication, recognition, and response to external stimuli. With rich experience, Creative Proteomics offers high-quality cell surface proteomics services, providing valuable insights into your protein research.

What is Cell Surface Proteomics

Cell surface proteins are molecular entities that reside on the outer membrane of cells, serving as key mediators of interactions between the cell and its external environment. These proteins play pivotal roles in various cellular processes, including signal transduction, adhesion, recognition, and immune response. The diversity of cell surface proteins reflects the functional specialization of different cell types and their ability to respond dynamically to extracellular cues.

Fig.1 Schematic of cell surface proteomics (Wojdyla, K., et al.; 2020)

Cell surface proteins exhibit a range of structural features to fulfill their specific functions. Many possess extracellular domains responsible for interactions with other cells, ligands, or the extracellular matrix. Transmembrane domains anchor these proteins in the lipid bilayer, connecting the extracellular and intracellular regions. Some cell surface proteins lack transmembrane domains and are instead anchored to the membrane via lipid modifications. Additionally, cell surface proteins may have intracellular domains that participate in signaling cascades or interactions with the cellular cytoskeleton.

Applications of Cell Surface Proteomics

The cell surface is a dynamic interface that mediates crucial interactions between cells and their environment, influencing processes such as signal transduction, adhesion, and immune response. Cell surface proteomics aims to identify, characterize, and quantify the diverse array of proteins residing on the cell surface, providing valuable insights into cellular communication, recognition, and response to external stimuli. It has broad applications in various fields, such as:

Our Service

With advanced protein detection technology, Creative Proteomics provides targeted solutions for researchers and scientists to help them utilize powerful analytical techniques to reveal the complexity of the proteome. With our thoughtful and detailed services, you can choose any protein analysis, including qualitative, quantitative, targeted and PTM analysis, and we will provide you with a detailed plan according to your requirements. The cell surface proteomics service process is as follows:

Fig.2 Cell surface proteomics workflow

• Sample Preparation
  Proper extraction and preparation of proteins from samples to ensure retention of cell surface proteins.
• Enrichment
  Enrichment techniques are used to isolate proteins or peptides from the cell surface. This step is critical because cell surface proteins are usually less abundant compared to total cellular proteins.
• Detection
  WB, protein microarrays and LC-MS/MS are powerful tools for the identification and quantification of peptides, and they provide detection information that facilitates a comprehensive analysis of the cell surface proteome.
• Bioinformatics Analysis
  The raw mass spectrometry data undergoes bioinformatics analysis to identify proteins, quantify their abundance, and infer potential functional roles.
• Result Delivery
  After professional data analysis, we deliver results quickly and consistently.

Creative Proteomics brings a wealth of experience to the field of protein research. Our comprehensive services and products cover the entire spectrum of utilizing suitable and efficient gateways. Utilizing our proprietary platform, we have successfully implemented many proteomics research projects. If you are interested, please contact us for more information and a quote.

Reference

• Wojdyla, K.; et al. Cell-Surface Proteomics Identifies Differences in Signaling and Adhesion Protein Expression between Naive and Primed Human Pluripotent Stem Cells. Stem Cell Reports. 2020, 14(5): 972-988