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DIA Quantitative Proteomics Analysis

How to efficiently determine proteins with low abundance in complex samples is an obstacle to protein research. DIA can provide an effective solution to this problem, as well as improve protein analysis' accuracy, confidence, and reproducibility. As a company that has specialized in proteomics research for many years, Creative Proteomics can provide professional DIA proteomics services to facilitate your protein research.

What Is DIA Quantitative Proteomics

In recent years, DIA (Data Independent Acquisition) has become one of the most popular MS data acquisition modes, enabling comprehensive and unbiased analysis of proteins in complex samples, leading to new developments in quantitative proteomics. It develops on the traditional DDA mode and allows efficient detection of very low abundance protein molecules in complex samples, greatly improving quantitative accuracy, reliability and reproducibility.

The Principle of DIA Proteomics

In proteomics, precursors are fragmented and analyzed by MS to generate the ion spectra of proteins' fragments in the sample. After primary MS, DDA collects precursors with high ionic intensities in the preset window and then sends them to secondary MS for fragmentation and analysis. Therefore, DDA can't effectively collect information about proteins with low abundance, and the collection is random in nature. It is less accurate and reproducible, and unsuitable for complex samples. In contrast, DIA splits all precursor ions by m/z into different windows, then sends all precursors in each window to secondary MS for fragmentation and analysis. In DIA, without relying on the choice of precursors, all ions in the whole m/z range are detected at regular intervals, in order to analyze proteins in complex samples comprehensively, accurately and reproducibly. DIA overcomes DDA's difficulties in accuracy and reproducibility, and it can detect proteins at very low levels, making it suitable for complex samples.

Schematic of DDA and DIA proteomics

Fig. 1. Schematic of DDA and DIA proteomics

Applications of DIA Quantitative Proteomics

DIA proteomics is becoming more and more popular due to its ability to provide more comprehensive quantitative data. It is applied to medicine, agriculture, the environment, food and other life science fields. And the applications include building bioinformatics libraries, discovering biomarkers, characterizing tumors and diseases, crop development and strain comparison, etc. And it can also be used to analyze complex biological samples. In summary, DIA proteomics can analyze samples with high quality and has a wide range of applications.

Our DIA Quantitative Proteomics Analysis Service

The DIA proteomics combines high-throughput and precise quantitative analysis, allowing the quantitative analysis of almost all detectable proteins in complex samples. Creative Proteomics provides considerate and high-quality DIA proteomics services. You just need to make your request and send us the samples, then we will take care of all the follow-up matters, including protein extraction, enzyme digestion, separation, MS analysis, data analysis, and bioinformatics analysis. In addition, a more advanced 4D-DIA quantitative proteomics analysis is also available.

Fig. 2. DIA Workflow

Fig. 2. DIA Workflow

Advantages of DIA Quantitative Proteomics Analysis

Creative Proteomics provides comprehensive and high-quality proteomics analysis services. If you are interested in DIA quantitative proteomics analysis, please contact us for more details.

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