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Label-free Quantitative Proteomics Analysis

In the realm of protein research, label-free quantitative proteomics analysis emerges as a useful approach, preserving the most authentic data from biological samples. Creative Proteomics, a company with a profound specialization in proteomics research spanning numerous years, stands ready to deliver top-tier label-free quantitative proteomics analysis services.

What is Label-free Proteomics

Label-free proteomics stands as an invaluable analytical technique for delving into proteomes through the utilization of LC-MS technology. This method enables the quantification of proteins by scrutinizing the MS data of peptides and contrasting their signal intensities across diverse samples. One of the most significant advantages of label-free proteomics is its cost-effectiveness. Unlike traditional proteomics techniques that rely on isotopic labeling, which can be expensive and cumbersome, label-free proteomics simplifies Researchers can analyze multiple biological samples without the need for specialized reagents or complex labeling procedures. This cost-effective nature makes label-free proteomics an attractive choice for laboratories with budget constraints. Moreover, label-free proteomics is highly adaptable. It accommodates a wide range of sample types and experimental setups, making it suitable for various research objectives. Whether investigating changes in protein expression under different conditions, studying the dynamics of protein-protein interactions, or conducting biomarker discovery, label-free proteomics provides a versatile and reliable platform for relative protein quantification.

The Principle of Label-free Quantitative Proteomics

By comparing the relative abundance of proteins in different samples without stable isotopes or chemical tags, label-free proteomics quantifies proteins. It determines the differences in protein expression levels by analyzing the samples through LC-MS/MS and quantifying the peptide signals of the corresponding proteins in different samples. Unlike traditional isotope label-based proteomics, it requires less pretreatment, does not require high concentrations, retains more original information, covers more proteins, even those with low abundance, and allows detection of large amounts of samples.

Fig. 1. Schematic of label-free proteomics

Fig. 1. Schematic of label-free proteomics (Megger, D. A., et al., 2013)

Applications of Label-free Quantitative Proteomics

Label-free proteomics is very economical and flexible, and suitable for many fields like medicine, agriculture, animal husbandry, the environment, food and nutrition. The applications include: drug mechanism research, characterizing disease, biomarker screening, drug target research, plant stress resistance research, screening of special functional proteins, animal and plant reproductive development mechanisms, crop improvement, food development, etc.

Our Service

Label-free proteomics analysis distinguishes itself by its notable advantages when compared to traditional isotope label-based proteomics techniques. One standout feature is the reduction in the need for extensive sample pretreatment. This streamlined approach simplifies sample preparation, saving valuable time and resources. It is a cost-effective method of quantitative analysis. Creative Proteomics proudly offers high-quality label-free quantitative proteomics analysis services. Additionally, we provide advanced 4D label-free quantitative proteomics analysis for those seeking even deeper insights.

Fig. 2. Label-free proteomics workflow

Fig. 2. Label-free proteomics workflow

Advantages of Label-free Quantitative Proteomics

Label-free quantitative proteomics offers several compelling advantages:

Creative Proteomics stands as your comprehensive source for top-tier proteomics analysis services. If label-free quantitative proteomics analysis aligns with your research interests, please do not hesitate to contact us for further details.

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