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Quantitative Proteomics Services

Dynamic changes in intracellular protein abundance play an important role in a variety of life processes. In our pioneering role in protein research, Creative Proteomics provides clients with a wide range of quantitative proteomics services.

What is Quantitative Proteomics?

Quantitative proteomics is the identification and quantification of all the proteins expressed by a genome or all the proteins in a complex mixed system, and the study of protein expression levels between different samples, conditions or times. Dynamic changes in intracellular protein abundance play an important role in a variety of life processes. For example, the onset and progression of many diseases are often accompanied by the abnormal expression of certain proteins. Quantitative protein studies help to gain insights into biological processes, disease mechanisms, drug responses, and more.

Fig.1. Schematic of quantitative proteomics

Fig.1. Schematic of quantitative proteomics

Development so far, quantitative techniques based on electrophoresis are gradually being replaced by quantitative techniques based on MS; which are highly sensitive, high throughput, and require less sample volume. The main quantitative proteomics techniques commonly used today are DDA and DIA data acquisition modes, Label and Label Free quantification strategies, and 4D separation techniques, etc. Among them, DIA is an upgrade to the DDA data acquisition mode, which can efficiently determine protein molecules with very low abundance in complex samples. The 4D separation technology enhances protein detection speed and coverage by adding ion mobility separation to traditional 3D separation. Label-free quantification strategy does not rely on expensive isotopes to relatively quantify proteins, while the label quantification strategy has higher sensitivity and accuracy than isotope labeling.

Our Services

Leveraging our extensive experience and expertise in the field of proteomics, Creative Proteomics offers a comprehensive suite of high-quality quantitative proteomics analysis services. These services are designed to address a wide range of research needs and objectives, providing valuable insights into protein abundance and dynamics. Our offerings include:

DIA Quantitative Proteomics Analysis

It develops on the traditional DDA mode and allows efficient detection of very low abundance protein molecules in complex samples, greatly improving quantitative accuracy, reliability and reproducibility.

4D-DIA Quantitative Proteomics Analysis

4D-DIA quantitative proteomics emerges as a robust methodology that marries the benefits of 4D separation technology with the DIA data acquisition mode. This synergy equips researchers to conduct thorough and in-depth investigations of complex proteomes, providing a holistic and precise portrait of the proteome.

Label-Free Quantitative Proteomics Analysis

Label-free quantitative proteomics enables quantitative analysis of proteins by scrutinizing MS data of peptides and comparing the signal intensities of different samples. It eliminates the need for expensive isotope labelling and is cost-effective for a wide range of biological samples.

4D Label-Free Quantitative Proteomics Analysis

4D label-free quantitative proteomics adds ion mobility separation to label-free quantitative analysis, enabling detecting low abundance protein signals hidden in 3D conditions. 4D separation technology combined with label-free quantification greatly enhances coverage, sensitivity, accuracy, and throughput of protein detection.

TMT-based Quantitative Proteomics Analysis

As a quantitative labeling technique, it employs isotope tags of equal mass to label the amino groups of peptides, allowing for the simultaneous comparison of protein expression levels across multiple samples. It compares up to 16 samples simultaneously with high accuracy and versatility.

iTRAQ-based Quantitative Proteomics Analysis

The iTRAQ-based approach is another widely embraced isotope labeling technique, characterized by tags that share a similar structure and quantification principle as TMT tags. Renowned for its precision and accuracy, it serves as an accurate method for both relative and absolute protein quantification.

SILAC-based Quantitative Proteomics Analysis

SILAC stands out as a robust and high-throughput methodology employed for protein quantification through the introduction of isotopic labeling during cell culture. Distinguished by its high protein labeling efficiency, SILAC maintains the integrity of protein quantification results even during the isolation and purification processes. This exceptional accuracy in labeling and quantification makes SILAC a preferred choice for researchers seeking dependable results in their studies.

Why Choose Us

Creative Proteomics provides comprehensive and high-quality proteomics analysis services. With highly specialized technical capabilities and mass spectrometry professionals, we are committed to assisting clients around the world in the fast-growing field of proteomics with fast turnaround and high-quality data at competitive prices. Our clients can contact our staff directly and get prompt responses to their inquiries. If you are interested in our services, please contact us for more details.

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