Qualitative Proteomics Services
The primary goal of qualitative proteomics is to gain insights into the protein composition of a sample, understand protein functions, and discover potential biomarkers or novel proteins of interest. In our pioneering role in protein research, Creative Proteomics provides clients with a wide range of quantitative proteomics services.
What is Qualitative Proteomics
In contrast to quantitative proteomics, qualitative proteomics is focused on the identification and analysis of protein sequences, profiles, and interactions between proteins and various substances. By examining and identifying proteins involved in different biological processes and disease states, qualitative proteomics provides a molecular foundation for disease research, enhances our understanding of the intricate structure and function of proteins, and reveals potential binding sites for drug development.
Fig.1. Schematic of quantitative proteomics
Proteins are highly diverse and functionally complex molecules, necessitating a range of characterisation studies. These studies encompass aspects like molecular weight, isoelectric point, primary structure identification, protein profiling, and protein interaction analysis. To achieve comprehensive protein characterization, scientists have developed various methods, including isoelectric focusing (IEF), shotgun proteomics, yeast two-hybrid assays, Edman degradation, co-immunoprecipitation (Co-IP), fluorescence colocalization, GST pull-down assays, BiFC (Bimolecular Fluorescence Complementation), LiP-MS (Limited Proteolysis-Mass Spectrometry), EMSA (Electrophoretic Mobility Shift Assay), DNase I footprinting assay, methylation interference, yeast one-hybrid assays, ChIP-Seq (Chromatin Immunoprecipitation Sequencing), RNA pull-down assays, CHIRP (Chromatin Isolation by RNA Purification), RIP (RNA Immunoprecipitation), protein microarrays, and more.
These techniques are developed based on electrophoresis or mass spectrometry (MS), some are used in conjunction with both electrophoresis and MS, and they find widespread applications in the field of protein characterization. Their use has significantly advanced the field of proteomics, driving progress in our understanding of proteins and their roles in various biological processes.
Our Service
Leveraging our extensive experience and expertise in the field of proteomics, Creative Proteomics offers a full suite of high-quality qualitative proteomics analysis services. These services are designed to meet a wide range of research needs and objectives, providing valuable insights into the systematic study of proteins. The services we offer include：
Protein Identification Service
There are nearly a million different types of proteins and they are very complex, so researching a target protein requires finding it in a sample first. Protein identification is the foundation of protein research. It is the identification of the protein mixture in a sample, including molecular weight, isoelectric point, primary structure, etc.
Protein Profiling Analysis Service
Protein profiling is the comprehensive analysis of proteins in biological samples and involves the systematic identification and characterization of the entire proteome in the sample. The goal is to gain insight into the types, quantities, modifications, interactions, and functions of proteins in a particular biological context. Shotgun proteomics could acquire large amounts of data in a short period of time with its high-throughput assays, making it an excellent choice for protein profiling analysis.
Protein Interaction Analysis Service
Proteins usually need to interact with other molecules to function. It is present in almost all biological processes such as transcription, translation, substance transport, signaling, biochemical reactions, etc. Therefore, it plays a vital role in medicine, biology and other research. With the accumulation of knowledge and technological advances, it has become easier and easier to study the interactions between proteins, nucleic acids, metabolites, etc., thus promoting the development of biotechnology and medicine. Protein interaction analysis mainly includes protein-protein interactions, protein-metabolite interactions, protein-DNA interactions and protein-RNA interactions.
Protein Microarray Analysis Service
Protein microarrays (or protein microarrays) are a high-throughput method for protein detection and functional studies, and there are two types of antibody microarrays and functional protein microarrays. Antibody microarrays have developed into one of the most powerful multiplex detection technologies. Functional protein microarrays, on the other hand, are more often applied to the study of protein interactions and functions. They have excellent performance in terms of speed, sensitivity and specificity, and can detect proteins qualitatively or quantitatively at high throughput, which is a powerful tool for protein quantitative analysis, protein identification, protein interaction analysis, post-translational modification and other studies. Protein microarray has a wide range of applications in biomarker detection, disease diagnosis, drug targeting and medical research. It can also be used in combination with MS to improve the breadth and depth of protein research.
Why Choose Us
Creative Proteomics provides comprehensive and high-quality proteomics analysis services. With highly specialized technical capabilities and mass spectrometry professionals, we are committed to assisting clients around the world in the fast-growing field of proteomics with fast turnaround and high-quality data at competitive prices. Our clients can contact our staff directly and get prompt responses to their inquiries. If you are interested in our services, please contact us for more details.
