Olink Proteomics Analysis Service

Olink Proteomics stands at the forefront of cutting-edge proteomics technologies, offering a unique approach to studying proteins through its Proximity Extension Assay (PEA) technology. With a wealth of expertise, Creative Proteomics extends the top-tier olink proteomics service to provide a valuable perspective on your protein-focused research.

What is Olink Proteomics

Olink Proteomics relies on PEA technology, which is capable of measuring multiple proteins simultaneously with high sensitivity and specificity. Each antibody pair of PEA is attached to a unique oligonucleotide probe, which is complementary in close proximity when the antibody binds to the target protein, forming a nucleotide chain. Using this dual recognition binding principle, PEA converts protein information into nucleic acid signals, greatly enhancing detection specificity and minimizing background noise. It accurately detects low-abundance proteins and can quantify up to 92 protein biomarkers simultaneously, and has become a leading method for multiplexed protein analysis.

Fig. 1 Schematic of PEA technology (Wik, L., et al.; 2021)

PEA is a targeted proteomics quantification method that combines antibody-based immunoassays with PCR or Next Generation Sequencing (NGS) technology. It begins by designing antibodies against target proteins so that complementary oligonucleotide probes are attached to each antibody. Then, after the antibody specifically binds to the target protein, the probe pairs on the antibody undergo proximity hybridization to form a PCR template, followed by amplification of the sample using primers. Finally, quantification is achieved by qPCR or NGS. Through this process, the protein concentration signal is converted to a nucleic acid signal, enabling the detection of low abundance proteins.

Applications of Olink Proteomics

Our Service

With rich proteomics experience and professional technology platform, Creative Proteomics provides customized services for researchers and scientists to help them use powerful immunoassay techniques and sequencing technologies to reveal the mysteries of specific proteins. Our services are thoughtful and meticulous, and the olink proteomics service process is as follows:

Fig 2. Workflow of olink proteomics

• Antibody Pair Design
  A unique pair of antibodies is designed for each target protein and ligated with oligonucleotide probes. Each pair is carefully selected and validated to ensure high specificity and sensitivity to the specific protein target.
• Sample Preparation
  Preparation of biological samples containing the target proteins to be tested. Olink proteomics is highly sensitive and specific, eliminating the need for deliberate removal of high-abundance proteins or enrichment of low-abundance proteins.
• Incubation
  Antibodies designed to capture specific proteins are used, and then the antibody-carrying probes are hybridized in close proximity to convert the protein information into a nucleic acid signal.
• Detection
  Nucleic acid signals in samples are analyzed using high-throughput techniques, which can usually be achieved by qPCR or NGS, and the resulting data can provide quantitative information on the abundance of multiple proteins in the sample.
• Bioinformatics Analysis
  Bioinformatics tools are often used to interpret and analyze the complex data generated by the assay. The final output consists of quantitative measurements of proteins in the sample, presented as normalized protein expression values.
• Result Delivery
  After professional data analysis, we deliver results quickly and consistently.

Creative Proteomics brings a wealth of experience to the field of protein research. Our comprehensive services and products cover the entire spectrum of utilizing suitable and efficient gateways. Utilizing our proprietary platform, we have successfully implemented many proteomics research projects. If you are interested, please contact us for more information and a quote.

Reference

• Wik, L.; et al. Proximity Extension Assay in Combination with Next-Generation Sequencing for High-throughput Proteome-wide Analysis. Molecular & Cellular Proteomics. 2021, 20: 100168