FFPE Tissue Proteomics
FFPE tissue proteomics is a specialized field that addresses the challenges posed by preservation methods commonly used in histopathology. With rich experience, Creative Proteomics offers high-quality FFPE tissue proteomics services, providing valuable insights into your protein research.
What is FFPE Tissue Proteomics
Formalin-fixed, paraffin-embedded (FFPE) tissue is a widely used method for preserving biological samples, especially in histopathology. This technique involves fixing tissues with formaldehyde to preserve cellular structures and prevent decay, followed by embedding them in paraffin wax for long-term storage. FFPE tissue is a valuable resource for retrospective studies, allowing researchers to analyze archived samples and gain insights into the molecular and pathological characteristics of various diseases.
Fig.1 Schematic of FFPE tissue proteomics (Buczak, K., et al.; 2020)
Due to its wide availability and long-term preservation of tissue structure and morphology, FFPE tissue is an invaluable resource for studying the underlying molecular mechanisms of disease. Isolation of specific regions from FFPE tissue and assessment of its protein heterogeneity by proteomic techniques allows for the dissection of genomic mutations, differential expression and signaling pathways in samples such as tumors. Thus, FFPE tissue proteomics has significant clinical implications, especially in biomarker discovery-driven studies.
Applications of FFPE Tissue Proteomics
Biomarker Discovery
FFPE tissue proteomics helps identify protein biomarkers associated with various cancers. These biomarkers can be crucial for early detection, prognosis, and treatment monitoring.
Disease Research
FFPE tissue proteomics studies protein expression patterns in diseased tissues and helps to understand the pathological mechanisms of disease.
Drug development
FFPE tissue proteomics assists in assessing the impact of drugs on tissue protein expression, aiding in toxicology studies during drug development.
Personalized medicine
Proteomic profiling of FFPE samples can contribute to patient stratification based on molecular characteristics, enabling more targeted and personalized treatment approaches.
Our Service
With advanced protein detection technology, Creative Proteomics provides targeted solutions for researchers and scientists to help them utilize powerful analytical techniques to reveal the complexity of the proteome. With our thoughtful and detailed services, you can choose any protein analysis, including qualitative, quantitative, targeted and PTM analysis, and we will provide you with a detailed plan according to your requirements. The FFPE tissue proteomics service process is as follows:
Fig.2 FFPE tissue proteomics workflow
- Sample Preparation
Accurate sampling of FFPE tissue samples is the basis for this analysis, which requires precise separation techniques and proper preservation. - Protein Extraction
Proteins isolated and extracted from FFPE tissue samples are a crucial step in FFPE tissue proteomics. - Detection
WB, protein microarrays and LC-MS/MS are powerful tools for the identification and quantification of peptides, and they provide detection information that facilitates a comprehensive analysis of FFPE tissue proteome. - Bioinformatics Analysis
The raw mass spectrometry data undergoes bioinformatics analysis to identify proteins, quantify their abundance, and infer potential functional roles. - Result Delivery
After professional data analysis, we deliver results quickly and consistently.
Creative Proteomics brings a wealth of experience to the field of protein research. Our comprehensive services and products cover the entire spectrum of utilizing suitable and efficient gateways. Utilizing our proprietary platform, we have successfully implemented many proteomics research projects. If you are interested, please contact us for more information and a quote.
Reference
- Buczak, K.; et al. Spatially resolved analysis of FFPE tissue proteomes by quantitative mass spectrometry. Nature Protocols. 2020, 15(9): 2956-2979