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Disulfide Bond Analysis Service

In proteins, disulfide bonds are key to changing their spatial structure and maintaining stability and functionality. Creative Proteomics offers superior disulfide bond analysis services to provide valuable insights into your protein research.

What is Disulfide Bond

Comprising a sulfhydryl group linking to 2 cysteine residues, -S-S- is characterized by its acknowledged stability. These bonds can form within the same peptide chain or between different peptide chains of proteins, playing a crucial role in folding, connecting, and stabilizing the spatial structure of peptide chains. Typically, an increase in the number of -S-S- correlates with higher protein stability against external factors. Nevertheless, these bonds are vulnerable to reduction, particularly in the reducing environment of the cytoplasm.

Fig.1 Schematic of disulfide bonds

Fig.1 Schematic of disulfide bonds (Hidaka, Y.; Shimamoto, S.; 2022)

Found extensively in proteins, -S-S- exert significant influence on both secondary and tertiary structures. Commonly occurring in antibodies, growth factors, hormones, and receptors, these bonds also play a pivotal role in various protein products and target proteins within the domain of biopharmaceuticals. This includes antibody-coupled drugs and peptide pharmaceuticals. Tailored analysis services dedicated to protein -S-S- ensure a thorough examination, yielding valuable insights into their impact on protein folding, stability, and function.

Applications of Disulfide Bond Analysis

Our Service

Creative Proteomics has advanced protein detection technologies and can provide customized services to researchers and scientists to help them reveal the complexity of PTM using powerful mass spectrometry technologies. Our services are thoughtful and detailed and the process of disulfide bond analysis service is as follows:

Fig.2 Disulfide bond analysis workflow

Fig.2 Disulfide bond analysis workflow

Creative Proteomics brings a wealth of experience to the field of protein research. Our comprehensive services and products cover the entire spectrum of utilizing suitable and efficient gateways. Utilizing our proprietary platform, we have successfully implemented many proteomics research projects. If you are interested, please contact us for more information and a quote.

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