Online Inquiry

PRM Targeted Proteomics Analysis

In the expansive realm of protein assays, the sheer diversity and abundance of data pose a significant challenge to swiftly and accurately pinpointing the protein of interest. The ability to precisely locate the target protein amid this vast protein landscape becomes paramount for influencing effective protein research. Enter PRM technology—an apt choice for this task. PRM excels at selecting the specific peptide of interest from the myriad of peptides, aiding researchers in the precise localization of their target protein. With a steadfast commitment to the domain of protein research over many years, Creative Proteomics offers meticulous and thoughtful PRM targeting analysis services. These services serve as a valuable ally for researchers, facilitating swift and precise penetration into the intricate world of protein studies.

What is PRM Targeted Proteomics

PRM (Parallel Reaction Monitoring), the current mainstream method for targeted proteomics data acquisition, achieves relative or absolute quantification of target proteins by selectively detecting specific peptides or target peptides. PRM is an upgraded version of MRM/SRM. The principle of screening target proteins in primary mass spectrometry is the same for both techniques, which is to screen precursor ions for the next step of detection through a four-stage rod, and the difference is the detection mode of secondary mass spectrometry. In secondary mass spectrometry, MRM/SRM detects specific fragment ions to quantify and characterize proteins in a one-to-one manner, while PRM detects all fragment ions. Thanks to the comprehensive detection mode of secondary mass spectrometry, PRM technology is much more sensitive and resistant to interference, as well as simpler and easier to use.

The Principle of PRM Targeted Proteomics

The principle of PRM technology is an ion detection technology based on high-resolution and high-precision mass spectrometry, which is capable of selectively detecting target proteins and target peptides, thus realizing the quantification of target proteins/peptides. The selective detection capability of the four-stage rod mass analyzer is first utilized to selectively detect the parent ion information of the target peptide in the primary mass spectrum. Subsequently, the parent ions are fragmented in a collision cell; finally, the high-resolution, high-quality precision analyzer is utilized to detect the information of all fragments within the window of the selected parent ions in the secondary mass spectrum. In this way, the target proteins/peptides in complex samples can be accurately and specifically analyzed. Shot-gun and PRM are two different approaches to mass spectrometry. Shot-gun is aimed at detecting as many proteins as possible in a sample, making it easy to detect high abundance proteins, whereas PRM is aimed at accurate quantification of specific proteins.

Fig. 1. Schematic of MRM/SRM and PRM data acquisition

Fig. 1. Schematic of MRM/SRM and PRM data acquisition

Applications of PRM Targeted Proteomics

PRM can be used for the validation of wide screening data, such as target proteins obtained from genomes, transcriptomes, quantitative proteomes, and modified proteomes, to achieve their relative/absolute quantitative analysis. PRM-targeted proteomics also has a range of applications, such as protein biomarker validation, post-translational modification studies, drug discovery, protein interaction studies, personalized medicine, food safety and environmental monitoring, and systems biology.

Our Service

Creative Proteomics extends a comprehensive suite of PRM technology services, encompassing the validation of specific protein changes in quantitative proteomics, both relative and absolute quantification of proteins, and the precise quantification of protein modification sites. If you find yourself in need of protein quantification but lack the necessary antibodies, our PRM targeted proteomics analysis stands as a reliable and effective choice. Moreover, for those seeking even greater accuracy and reliability, we offer the advanced 4D-PRM targeted proteomics analysis service.

Fig. 2. PRM targeted proteomics workflow

Fig. 2. PRM targeted proteomics workflow (Park J, et al., 2020)

Advantages of PRM Targeted Proteomics Analysis

Creative Proteomics provides comprehensive and high-quality proteomics analysis services. If you are interested in PRM targeted proteomics analysis service, please contact us for more details.

Reference

5-20% Discount 5-20% Discount