Single-cell Proteomics
Single-cell proteomics aims to characterize the protein expression profiles of individual cells with high precision, overcoming the limitations of traditional approaches that may mask heterogeneity at the level of individual cells, and holds great promise for understanding cellular diversity, unraveling rare cell populations and deciphering the mysteries of complex biological systems. With rich experience, Creative Proteomics offers high-quality single-cell proteomics services, providing valuable insights into your protein research.
What is Single-cell Proteomics
The single-cell proteome refers to the entire complement of proteins present within an individual cell. Analyzing the proteome at the single-cell level has become a pivotal focus in the field of proteomics, as it allows researchers to uncover unique protein expression profiles and heterogeneity among individual cells within a population. This level of precision is particularly crucial in understanding diverse cellular functions, identifying rare cell types, and gaining insights into the molecular intricacies that govern cellular behavior.
Fig.1 Schematic of single-cell proteomics (Goto-Silva, L., Junqueira, M.; 2020)
It involves the isolation, characterization, and quantification of proteins from individual cells. Techniques such as microfluidics, single-cell mass spectrometry, and high-throughput immunoassays enable the isolation of proteins from a single cell while maintaining a spatial and temporal context. These approaches address the challenges posed by cellular heterogeneity and reveal nuanced variations in protein expression among individual cells. Sensitivity and specificity are paramount in single-cell proteomics to capture the intricacies of low-abundance proteins and subtle changes in cellular states.
Applications of Single-cell Proteomics
Single-cell proteomics not only provides a comprehensive view of cellular heterogeneity but also offers insights into the molecular basis of diseases and potential avenues for therapeutic intervention. The applications of single-cell proteomics span various fields, such as:
Developmental Biology
In developmental biology, studying the single-cell proteome helps unravel the molecular events underlying cell differentiation and tissue formation.
Cancer Research
In cancer research, identifying unique proteomic signatures at the single-cell level allows for a deeper understanding of tumor heterogeneity and the identification of potential therapeutic targets.
Immunology
In immunology, analyzing the single-cell proteome contributes to deciphering the diversity of immune cell populations and their responses to stimuli.
As technology continues to advance, single-cell proteomics holds the promise of uncovering previously hidden facets of cellular biology, paving the way for more targeted and personalized approaches in medicine and biotechnology.
Our Service
With advanced protein detection technology, Creative Proteomics provides targeted solutions for researchers and scientists to help them utilize powerful analytical techniques to reveal the complexity of the proteome. With our thoughtful and detailed services, you can choose any protein analysis, including qualitative, quantitative, targeted and PTM analysis, and we will provide you with a detailed plan according to your requirements. The single-cell proteomics service process is as follows:
Fig.2 Single-cell proteomics workflow
- Single Cell Screening
Single cell screening is the basis of single-cell proteomics, using microfluidic microarrays, FACS and other methods to accurately screen individual cells from samples. - Protein Extraction
After screening the single cells, extract the proteins and detect them. - Detection
WB, protein microarrays and LC-MS/MS are powerful tools for the identification and quantification of peptides, and they provide detection information that facilitates a comprehensive analysis of the single-cell proteome. - Bioinformatics Analysis
The raw mass spectrometry data undergoes bioinformatics analysis to identify proteins, quantify their abundance, and infer potential functional roles. - Result Delivery
After professional data analysis, we deliver results quickly and consistently.
Creative Proteomics brings a wealth of experience to the field of protein research. Our comprehensive services and products cover the entire spectrum of utilizing suitable and efficient gateways. Utilizing our proprietary platform, we have successfully implemented many proteomics research projects. If you are interested, please contact us for more information and a quote.
Reference
- Goto-Silva, L.; Junqueira, M. Single-cell proteomics: A treasure trove in neurobiology. Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 2021, 1869(7): 140658