N-glycosylation Analysis Service

Protein N-glycosylation is a prevalent glycosylation modification crucial for tissue formation and implicated in various life processes, including cell recognition, immune response, and cell differentiation. At Creative Proteomics, we specialize in delivering high-quality N-glycosylation analysis services, which encompass the identification, quantification, and characterization, providing valuable insights for your protein research endeavors.

What is N-glycosylation

Protein N-glycosylation is a foundational post-translational modification where complex oligosaccharide chains covalently attach to specific asparagine residues in the consensus sequence Asn-X-Ser/Thr (with X being any amino acid except proline). This highly regulated and essential process unfolds in the endoplasmic reticulum and Golgi apparatus, playing a pivotal role in modulating protein folding, stability, and function.

Fig.1 Schematic of N-glycosylation (Esmail, S.; Manolson, M. F. 2021)

The initiation of N-glycosylation involves the en bloc transfer of a preassembled oligosaccharide precursor, typically a 14-sugar unit, to the nascent polypeptide chain. This initial step transpires in the endoplasmic reticulum, with subsequent modifications occurring in the Golgi apparatus. The attached oligosaccharides undergo further processing, including trimming and the addition of more complex sugar structures. The resulting glycan structures contribute significantly to the diversity of N-glycosylated proteins. N-glycosylation is critical for proper protein folding and quality control, influencing protein-protein interactions and impacting the recognition of glycoproteins by receptors.

Applications of N-glycosylation Analysis

• Cell Signaling Study
  Protein N-glycosylation has profound implications for cellular functions, including immune response, cell adhesion, and signaling.
• Disease Research
  Alterations in N-glycosylation patterns are associated with various diseases, including cancer. The identification of specific glycan signatures on proteins has potential diagnostic and prognostic applications in disease detection.
• Drug Development
  Therapeutic proteins produced in biotechnological processes, such as monoclonal antibodies, are often engineered to ensure appropriate N-glycosylation, as it can significantly influence their efficacy and safety.

Our Service

Creative Proteomics has advanced protein detection technologies and can provide customized services to researchers and scientists to help them reveal the complexity of PTM using powerful mass spectrometry technologies. Our services are thoughtful and detailed and the process of N-glycosylation analysis service is as follows:

Fig.2 N-glycosylation analysis workflow

• Sample Preparation
  Proper extraction and preparation of proteins from biological samples to ensure preservation of N-glycosylated proteins.
• N-glycosylation Enrichment
  Enrichment techniques are employed to isolate N-glycosylated proteins or peptides. This step is crucial as N-glycosylation modifications are often present in low abundance compared to unmodified proteins.
• Detection
  WB, protein microarrays and LC-MS/MS are powerful tools for the identification and quantification of N-glycosylated peptides, and they provide detection information that facilitates a comprehensive analysis of N-glycosylation.
• Bioinformatics Analysis
  Advanced bioinformatics tools are used to analyze data, identify N-glycosylated peptides, and map these modifications to protein sequences.
• Result Delivery
  After professional data analysis, we deliver results quickly and consistently.

Why Choose Us

• Rich experience in proteomics research
• Excellent research team and professional platform
• Unique and comprehensive research services in proteomics field
• Reliable data and results

Creative Proteomics brings a wealth of experience to the field of protein research. Our comprehensive services and products cover the entire spectrum of utilizing suitable and efficient gateways. Utilizing our proprietary platform, we have successfully implemented many proteomics research projects. If you are interested, please contact us for more information and a quote.

Reference

• Esmail, S.; Manolson, M. F. Advances in understanding N-glycosylation structure, function, and regulation in health and disease. European Journal of Cell Biology. 2021, 100(7): 151186