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N-glycosylation Analysis Service

Protein N-glycosylation is a prevalent glycosylation modification crucial for tissue formation and implicated in various life processes, including cell recognition, immune response, and cell differentiation. At Creative Proteomics, we specialize in delivering high-quality N-glycosylation analysis services, which encompass the identification, quantification, and characterization, providing valuable insights for your protein research endeavors.

What is N-glycosylation

Protein N-glycosylation is a foundational post-translational modification where complex oligosaccharide chains covalently attach to specific asparagine residues in the consensus sequence Asn-X-Ser/Thr (with X being any amino acid except proline). This highly regulated and essential process unfolds in the endoplasmic reticulum and Golgi apparatus, playing a pivotal role in modulating protein folding, stability, and function.

Fig.1 Schematic of N-glycosylation

Fig.1 Schematic of N-glycosylation (Esmail, S.; Manolson, M. F. 2021)

The initiation of N-glycosylation involves the en bloc transfer of a preassembled oligosaccharide precursor, typically a 14-sugar unit, to the nascent polypeptide chain. This initial step transpires in the endoplasmic reticulum, with subsequent modifications occurring in the Golgi apparatus. The attached oligosaccharides undergo further processing, including trimming and the addition of more complex sugar structures. The resulting glycan structures contribute significantly to the diversity of N-glycosylated proteins. N-glycosylation is critical for proper protein folding and quality control, influencing protein-protein interactions and impacting the recognition of glycoproteins by receptors.

Applications of N-glycosylation Analysis

Our Service

Creative Proteomics has advanced protein detection technologies and can provide customized services to researchers and scientists to help them reveal the complexity of PTM using powerful mass spectrometry technologies. Our services are thoughtful and detailed and the process of N-glycosylation analysis service is as follows:

Fig.2 N-glycosylation analysis workflow

Fig.2 N-glycosylation analysis workflow

Why Choose Us

Creative Proteomics brings a wealth of experience to the field of protein research. Our comprehensive services and products cover the entire spectrum of utilizing suitable and efficient gateways. Utilizing our proprietary platform, we have successfully implemented many proteomics research projects. If you are interested, please contact us for more information and a quote.

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