Good, active, effective antibodies are a multi-billion dollar industry. It is not enough to have hybridoma cells alone, because storage accidents, genetic drift or contamination can threaten the safety of antibodies. Sequence analysis of the antibody is therefore required so that recombinant production of the antibody can be carried out at any time. Or if you want to protect the property rights of the antibody or expand the culture, it is very necessary to obtain the accurate sequence information of the antibody. Sequence analysis of antibodies is the starting point for protecting your antibodies and building antibody engineering programs, such as isotype and subtype switching, and even antibody humanization.
Mass spectrometry-based Sequence Analysis of Monoclonal Antibodies
It was too late to sequence the hybridomas because the cell lines had been lost or unavailable. With the continuous development of mass spectrometry technology, antibody sequencing can be achieved as long as there is a sufficient amount of antibodies; monoclonal antibody molecules play a key role in drug development and diagnostic reagent development. The primary structure of the antibody molecule itself, especially the amino acid sequence of its CDR region, is the core of its biological function, and the accurate and rapid analysis of its complete sequence is of great significance.
Antibody Sequencing Service
Based on the Obitrap Fusion Lumos mass spectrometry instrument with the highest resolution in the world, and combined with rich experience in bioinformatics analysis, Creative Proteomics has established a new generation antibody sequencing platform, which can realize fast and accurate analysis of antibody primary structure. The platform is suitable for sequence analysis of different subtypes of antibodies (eg IgG and IgM) and different types of antibodies (fluorescence-conjugated antibodies, immobilized antibodies, antibodies of different species). The antibody sequencing service of Creative Proteomics can analyze the full-length sequence of the antibody and the amino acid sequence of the variable region, and obtain the complete sequence information of the light chain and heavy chain of the antibody for customers.
Related services
Full Length protein/Antibody Sequencing
C-terminal Lysine Variants Determination
Creative Proteomics' antibody sequencing services are performed independently by our lab, ensuring the security of your intellectual property.
Advantages of Our Antibody Sequencing Service
Full-sequence analysis: Optimized high-resolution mass spectrometry workflow and best-in-class antibody de novo sequencing software ensure full-sequence analysis of the entire antibody from the N-terminus to the C-terminus;
Unparalleled accuracy: the sequence of each amino acid is derived from multiple MS/MS mass spectral data of multiple peptides;
Broad applicability: The technology is applicable to all antibody types (IgG, IgA, IgM, IgY, Fab, ScFv, etc.) and species (human, mouse, rat, rabbit, hamster, etc.). We can also sequence conjugated and contaminating proteins and antibodies with two different light chains.
High-throughput: Whether sequencing one antibody or hundreds of antibodies, our sequencing services make it possible.
Sample requirement
Monoclonal antibodies >50-100 ug
