The analysis of peptide mass fingerprinting technology on biological therapeutic drugs can achieve high-quality and effective identification and analysis of protein structural stability. Peptide mass fingerprint technology plays an important role in the study of molecular structure characterization and analysis of macromolecular biological substances such as hormones, antibiotics, proteins and new drugs. We can provide peptide mass fingerprint technology for protein characterization information analysis. For example, the protein after digestion can be analyzed by different types of mass spectrometry, such as using mass spectrometer ESI-TOF or MALDI-TOF combined with MS/MS method, which can analyze multiple proteins in one experiment to achieve comprehensive protein structure analysis research.
Creative Proteomics is a reliable biopharmaceutical partner with professional proteomics research resources. We use first-class mass spectrometry technology experiment platform and mature technical methods to provide you with high-quality one-stop service. Proteomics and structural characterization information analysis of biotherapeutic drugs are key components of quality control in the biopharmaceutical industry. In particular, the ICH Q6B guidelines require the provision of sequence information on protein structures of biopharmaceuticals. We will provide you with comprehensive and GLP / cGMP-compliant N-terminal sequencing analysis service around the ICH guidelines (especially ICH Q6B) and the US FDA issues 'Points to Consider' document.
We Can Provide but Not Limited to:
➢ Protein molecular weight analysis
➢ Advanced structural analysis of proteins
➢ Identification of unknown protein types
➢ Target peptide quality analysis
➢ Peptide mass fingerprint image analysis
➢ Analysis of protein modification sites
Technology Platform of Peptide Mass Fingerprinting Analysis Service:
Creative Proteomics provides ESI-TOF or MALDI-TOF peptide mass fingerprinting methods including gel staining method, peptide fragment extraction, concentration, desalting, soft ionization technology, spectrum collection and other technologies to analyze protein structure characterization information.
The analysis process of peptide mass fingerprint technology includes protein extraction, proteolytic cleavage, and the absolute mass of the target peptide using a mass spectrometer such as MALDI-TOF or ESI-TOF. It can be obtained by using a computer program to obtain the alignment of the theoretical peptides in known protein sequence databases, and according to the results of the data comparison to get the analysis of peptide mass fingerprint and protein identification. The specific workflow is as follows.
Advantages of Peptide Mass Fingerprinting Analysis Service:
➢ Short time-consuming: The service simplifies the use of a first-class experimental platform and sample preparation and detection steps. There is no need to sequence unknown peptides, only the quality of the peptides needs to be known, which greatly shortens the time for protein structure identification and analysis.
➢ High sensitivity: The identification of each peptide is based on its mass and MS/MS fingerprint, which is more accurate than using only first-order mass spectrometry, mainly because it can eliminate concerns about quality degradation.
➢ High accuracy: Tandem mass spectrometry (MS/MS) is used to measure fragment mass spectra and identify proteins, and the smaller and more uniform peptide fragment analysis methods are fast and accurate.
➢ High-throughput: In this service, the combination of specific and non-specific proteases can ensure 100% coverage of any digested protein, and can identify thousands of different proteins, while providing customers with more complete analysis data.
➢ Rapid turnaround time: 5-7 days to provide detailed technical reports.
➢ Customized service: We can customize professional solutions for you according to your research plan needs. You can select or suggest the required items for analysis.
Creative Proteomics's professional researchers can provide customers with reliable information on protein therapeutics to achieve breakthroughs in protein physical and chemical properties analysis and structural analysis. We will provide you with detailed experimental technical solutions as well as the final peptide map information analysis report and other bioinformatics analysis reports. We are committed to providing you with professional services in peptide mass fingerprints of biotechnology drugs to help you solve problems related to analytical technology.
References
- Alastair Aitken. Protein Identification by Peptide Mass Fingerprinting// Mass Spectrometry Data Analysis in Proteomics. Humana Press, 2007.
- Alty L T, Lariviere F J. Peptide mass fingerprinting of egg white proteins. Journal of Chemical Education, 2016, 93(4): 772-777.
