What is NMR spectroscopy?
Nuclear magnetic resonance (NMR) spectroscopy is an analytical technique based on the magnetic properties of certain atomic nuclei. It depends on the phenomenon of nuclear magnetic resonance. Nuclear magnetic resonance is based on the nuclear spin (I) properties. Generally there are several I values, but the nuclei obtaining I = 1/2 is most used in NMR spectroscopy. NMR spectroscopy may provide structural information of molecules, as well as properties of dynamics and reaction state.
What is NMR spectroscopy used for?
NMR has been the dominant method for biological products analysis in pharmaceuticals. It can be used in structural analysis, bioprocess validation and impurity profiling of biologics products including antibodies, recombinant proteins and peptides. NMR spectroscopy is usually performed depending on various nuclei (1H, 13C, 19F and 31P). In addition, both 1D and 2D NMR experiments can be done to obtain more detailed structural information.
Scientists from Creative Proteomics are experienced in NMR spectroscopy analysis and Creative Proteomics can provide professional assistance for structural information of biopharmaceutical products by using NMR spectroscopy.
Several tips should be noticed for protein analysis:
- The proteins should be in its native conformation.
- The proteins are preferred to be less than 30 kDa for high-resolution tertiary structure determination.
- The prepared sample typically contains more than 10 mg/ml of purified protein (> 95%) in the suitable buffer.
- The proteins used for NMR spectroscopy analysis needs to be stable for at least several days.
Creative Proteomics provides NMR spectroscopy services including:
- high-resolution structure determination and target protein construct verification
- validation of peptides, hormones and other metabolite
- ligand binding assay and fragment screening kinetics of biopharmaceutical products
