In thin layer chromatography (TLC), a solid phase, the absorbent, is a dry, finely powered material (usually silica gel, aluminium oxide (alumina), or cellulose) applied as a thin layer onto a solid support, such as a glass plate, a plastic foil or an aluminum sheet. The mobile phase, the solvent, is drawn up and move across the surface by capillary action. Different analytes move on the TLC plate at different rates, depending upon the nature of absorbent, solvent and analytes, and the chromatographic separation is achieved. Therefore, the solid phase material and solvent system for analysis should have extreme selectivity toward the substances being separated. Furthermore, high performance thin layer chramotagraphy (HPTLC) has been developed, allowing a fast separation and flexible application.
Creative Proteomics offers TLC and HPTLC for pharmaceutical analysis with wide applications upon your demands. With a relatively high degree efficiency for separation, the target and unwanted impurities can be separated and then detected by different staining and visualization methods. A quantitative scanning is also available. A further quantification can be done by spot elution followed by spectrophotometric measurement, GC, GC-MS, or HPLC. In this way, the unknown or related compounds in bulk drugs can be fast screened and analyzed with a low cost.
