Isotopic labeling is a technique used to track the target molecule through a reaction, metabolic pathway, or signaling pathway. The target molecule is labeled with specific isotopes instead of its own atoms. In isotopic labeling assay, there are several ways for labeling isotopes determination, such as mass change, radioactive decay and vibrational mode. Depending on the labeling categories, isotopic trace can be subdivided into stable isotope labeling and radioactive isotope labeling.
Stable Isotope Labeling
Stable isotope-labeled proteins have been used in pharmacokinetic studies of biopharmaceuticals depending on the combination of labeling and mass spectrometry. Some of the most common stable isotopes are 2H, 13C, and 15N, which can further be produced into amino acids, peptide and proteins.
Radioactive Isotope Labeling
Radioactive labeling is a useful technique in biological drugs. This technique usually involves an isotope produced by radioactive decay. The common radioactive isotopes used in labeling are 125I, 99mTc, 3H, 14C and 35S. Among them, 125I is a popular radioactive isotope depending on high radioactivity, proper half-life and easy labeling. However all labeled proteins should be used as soon as possible due to radioactive decay.
Isotopic labeling assay service
Our special laboratory for isotope labeling is equipped with international first-class, complete and large-scale analytical and testing instruments and equipment for the analysis and separation of radioisotopes and stable isotope labeling compounds. Whether you are conducting quantitative proteomics or metabolite analysis studies, we can provide flexible and customized services to meet your diverse isotope labeling needs.
Our service include the following lists, but are not limited to:
- Specific activity and radiochemical purity analysis (radioisotope labeled compounds)
- Purity and abundance analysis (stable isotope labeled compounds)
- Preparative isolation and purification
- Analytical and assay studies in cells and solutions
- Related analysis and assays, and studies
- Storage and management of radiolabelled compounds for clients
- Providing comprehensive stable isotope labeling products, such as labeled amino acids, peptides, lipids, nucleic acids, and so on.
Detection platform
- γ radioisotope labeling and detection
- Beta radioisotope labeling and detection
- Stable isotope labeling and detection
- UPLC-MS
- Q-TOF LC-MS
- 1H-NMR (13C, 19F, 15N and 31P-NMR as required)
Applications of our service
- Small molecule drug detection
- Biological macromolecules such as peptides, polysaccharides, proteins, RNA and DNA detection
- Nanoparticle analysis and detection
Creative Proteomics provides a wide range of isotopic labeling assays for pharmacokinetic assays. Our scientists are experienced and can provide customer-tailored service. Please contact us if you have any questions regarding our services. We look forward to working with you on your next projects!
