The analysis of protein high-level structure is conducive to understanding the function of protein, and also conducive to understanding how protein performs its function. PEGylated proteins have the characteristics of poor modification reaction specificity due to the diversity of protein types and structural complexity. Therefore, the use of related technologies to analyze the advanced structural features of PEGylated proteins can reduce the blindness of PEG modification. At the same time, it can improve the efficiency of protein function research. At the same time, analyzing the structure, function and relationship of PEGylated protein is an important part of the proteome project.
Creative Proteomics has experienced experts in the field of protein drug research and a first-class experimental platform. We are committed to becoming a partner in the field of biopharmaceutical research. We will use advanced experimental technology and equipment to build an experimental bridge for you, and use the one-stop service concept to help you quickly complete experimental research and development projects. Advanced structural analysis of PEGylated proteins is an important part of ICH Q6B specification. We will provide you with PEGylated protein advanced structure analysis services around the ICH guidelines (especially ICH Q6B) and the US FDA issues 'Points to Consider' document.
We Can Provide but Not Limited to:
- Protein secondary structure analysis
- Advanced protein structure analysis
- Protein molecular mass analysis
Technology Platform of PEGylated Protein Advanced Structure Analysis Service:
Creative Proteomics uses hydrogen deuterium exchange mass spectrometry (HDX-MS), circular dichroism far ultraviolet scanning(CD-FUS) and X-ray crystal diffraction technology to analyze the advanced structure of PEGylated proteins efficiently and accurately.
We provide the following analysis methods:
PEGylated protein advanced structure analysis
1) Hydrogen deuterium exchange mass spectrometry
This technology mainly uses the hydrogen and deuterium atoms of the protein to discover the exchange phenomenon, and through mass spectrometry to determine the hydrogen and deuterium exchange rate of different sequence fragments of the protein, so as to obtain protein spatial structure information. The specific workflow is as follows:
2) Circular dichroism far ultraviolet scan
This technology is mainly to analyze the ratio of β-sheets and irregular curls in the secondary structure of the protein, as well as the UV scanning technology to obtain the distribution information of the chromophore groups and other residues in the side chain of the protein and the change of disulfide bonds, so as to obtain the structural characterization information of the protein.
3) X-ray crystal diffraction technique
This technology mainly uses the uniqueness of the X-ray light source. When the protein crystal is irradiated by X-rays, diffracted light waves will appear. The position information of the atoms in the protein crystal can be obtained according to the position and intensity of each spot in the X-ray diffraction image. The high-level structural information of protein macromolecules can be inferred from the diffraction image of the crystal.
Advantages of PEGylated Protein Advanced Structure Analysis Service:
- Short time-consuming: The service has a first-class experimental technology platform, which can reduce sample processing time and speed up the progress of experimental analysis.
- High quality: The service has a small demand for samples and can realize the test of experimental repeatability.
- High accuracy: Hydrogen-deuterium exchange mass spectrometry technology can provide accurate protein spatial structure characterization information.
- Rapid turnaround time: 5-7 days can provide customers with accurate and comprehensive experimental reports.
- Customized service: We can provide you with one-stop service according to your needs, and you can also choose the experimental plan you need.
Creative Proteomics's research team can provide customers with complete and comprehensive information on the high-level structure characterization of PEGylated proteins, including detailed experimental plans, related mass spectrometry diagrams, and final protein structure analysis reports and other experimental reports. We sincerely provide you with PEGylated protein-related analysis services to assist you in completing the research and development of protein-related experiments as soon as possible.
References
- Benhaim M, Lee KK, et al. Tracking Higher Order Protein Structure by Hydrogen-Deuterium Exchange Mass Spectrometry. Protein Pept Lett. 2019.
- Meisburger SP, Thomas WC, et al. X-ray Scattering Studies of Protein Structural Dynamics. Chem Rev. 2017.
