PEGylated protein has the unique advantage of prolonging the half-life of protein drugs, and has a significant effect on the biological activity of protein drugs in vivo. For this reason, it can improve the pharmacokinetics and pharmacodynamic properties of protein drugs. Has a key role. For this reason, the identification of PEGylated proteins is a key step in the quality control and pharmacokinetic research of biotechnology drugs.
Creative Proteomics has diversified PEGylated protein analysis technologies, which can solve important research and development problems in the quality control and pharmacokinetic research of biotechnology drugs. We are committed to working with you to become the most reliable ally in the development of biotechnology drugs, and to provide you with accurate and comprehensive product analysis reports. The identification and analysis of polyethylene glycol modified protein is one of the important components of protein drug quality control and biological safety analysis. In particular, the ICH Q6B guideline requires information on the identification and analysis of PEGylated proteins for protein drugs. We will provide you with PEGylated protein identification analysis services around the ICH guidelines (especially ICH Q6B) and the US FDA issues 'Points to Consider' document.
We Can Provide but Not Limited to:
Technology Platform of PEGylated Protein Identification Service:
Creative Proteomics uses ESI Q-TOF mass spectrometry, liquid-mass spectrometry and capillary electrophoresis technology to effectively and accurately identify and analyze PEGylated proteins.
We provide the following analysis methods:
PEGylated protein identification analysis
1) ESI Q-TOF mass spectrometry analysis
The analysis method includes two steps: In Source Fragmentation (ISF) and MS/MS. In the first ISF process, the PEG part of the PEGylated polypeptide is cleaved and shortened; in the second MS/MS process, the polypeptide is broken to produce b and y ion fragments. By analyzing the b and y ion information of the shortened PEG chain, the exact PEGylation modification site is finally obtained. The specific workflow is as follows:
2) LC/MS and capillary electrophoresis technology
This technology can complete PEGylated protein relative molecular weight determination, PEGylated protein sequence analysis, peptide mapping analysis, disulfide bond analysis, etc., and can perform quantitative and qualitative analysis of PEGylated protein.
Advantages of PEGylated Protein Identification Service:
- Short time-consuming: The service uses mature technology and advanced equipment to quickly complete the sample analysis process.
- High quality: The ESI Q-TOF mass spectrometry analysis technology used in this service can increase the speed and dynamic range of sample analysis, and the ultra-high resolution can determine the molecular weight of the intact protein, which can provide extremely reliable analysis results.
- High sensitivity: When processing complex low-abundance samples, the technical system of this service has the characteristics of ultra-high resolution, which can improve the sensitivity of sample analysis.
- Rapid turnaround time: 5-7 days can provide customers with accurate and comprehensive experimental reports.
- Customized service: We can provide you with tailor-made services based on your needs, and you can also choose your own analysis solutions.
Creative Proteomics has professional protein drug research and development experts, especially in the field of PEGylated protein related analysis, and can provide you with high-quality experimental analysis reports, including comprehensive and accurate experimental design plans, and related PEGylated protein mass spectra. Analysis results and other experimental reports. We are committed to solving technical problems related to proteomics for you to accelerate the marketization of protein function research and applications.
References
- Abdellatif AAH. Identification of somatostatin receptors using labeled PEGylated octreotide, as an active internalization. Drug Dev Ind Pharm. 2019.
- Mero A, Spolaore B, et al. Transglutaminase-mediated PEGylation of proteins: direct identification of the sites of protein modification by mass spectrometry using a novel monodisperse PEG. Bioconjug Chem. 2009.
