Creative Proteomics delves into the concept of N-terminal sequencing, explores the methods employed, discusses the applications of peptide mapping, and highlights the comprehensive services.
Confirming your protein's sequence and checking the termini for potential variations are regulatory requirements (see ICH Q6B guidelines section 6.1.1 c).
What is N-Terminal Sequencing?
The process known as N-terminal sequencing comprises the deciphering of the amino acid present at the N-terminus, essentially the initiation site, within a polypeptide or protein chain. The cruciality of delineating the N-terminal sequence stems from its integral role in delineating post-translational modifications, intra-protein interactions, and the intrinsic biological role of the protein in question. A thorough examination of the particular amino acid sequence provides a platform for insightful understanding of both the protein's structural and functional attributes, fundamental information for anyone involved in comprehensive protein research.
Fig 1. Identification and Sequencing of N‑Terminal Peptides in Proteins by LC-Fluorescence-MS/MS. (Vecchi, M. M., et al.; 2019)
N-terminal sequencing constitutes an important methodology for the identification of the initial amino acid constituting the N-terminus of a peptide or protein chain. Elucidating the N-terminal sequence is vital in comprehending key biological mechanisms, including post-translational modifications, protein-protein interaction dynamics, and overall protein functionality. This dissection of the precise amino acid sequence facilitates the exploration of intricate protein structure and function, thereby contributing to our understanding of the protein of interest.
Methods of N-Terminal Sequencing
N-terminal sequencing encompasses several methods, the widely employed technique is Edman degradation, which involves the incremental removal of the N-terminal amino acid residue, followed by identification and quantification. This process consists of four key steps:
- Pre-activation
- Edman degradation
- Cleavage and detection
- Repeated cycles
Fig 2. Workflow for identification and sequencing of N-terminus by mass spectrometry. (Vecchi, M. M., et al.; 2019)
Application of N-Terminal Sequencing
Post-Translational Modifications
Protein-Protein Interactions
Protein Engineering and Design
Quality Control of Protein-based Therapeutics
Structural Analysis
Proteomic Studies
Our Services
Creative Proteomics specializes in providing comprehensive N-terminal sequencing services, leveraging state-of-the-art techniques and expertise cultivated over 20 years. Our highly skilled scientists ensure the delivery of reliable and accurate results to our clients. Here are some of the key services we provide:
- Peptide Mapping and Structural Analysis
- High-Throughput and Efficiency
- Customized Solutions and Consultation
- Data Interpretation and Bioinformatics Analysis
- Quality Control and Validation
Services Process
Our Advantages
- Undertaking consistent financial ventures in state-of-the-art analytical equipment and methodology guarantees the production of precise and correct N-terminal sequencing results.
- Comprehending the quantum singularity of every protein, we engage in profound collaboration with our clientele to modify our services according to their distinctive requisites.
- The constituents of our team are seasoned scientists equipped with copious proficiency and specialization in the remarkable field of protein characterization.
- We operate through a systematic workflow coupled with proficient procedures, which not only ensures a swift turnaround time but also upholds the superior quality of the outcomes. Continuous investment in advanced analytical instruments and techniques ensures the generation of accurate and precise N-terminal sequencing results.
Our Commitment and Future Goals
At Creative Proteomics, we hold substantial proficiency in N-terminal sequencing and peptide mapping, providing an extensive variety of services to support scholars in their protein characterization research. Our avant-garde methodologies, tailor-made solutions, team of seasoned experts, and dedication to punctuality set us as the favored choice for researchers in need of N-terminal sequencing provisions. Should you possess an interest in our offerings or require further details or clarifications, please do not hesitate to initiate a correspondence with us. We ardently anticipate the opportunity to collaborate with you on innovative and significant research undertakings.
Reference
- Vecchi, M. M., et al.; Identification and sequencing of N-terminal peptides in proteins by LC-Fluorescence-MS/MS: an approach to replacement of the Edman degradation. Analytical Chemistry. 2019.
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For research use only, not intended for any clinical use.
