Protein activities and regulatory mechanisms can be better understood by analyzing post-translational changes, which may be crucial in areas such as the life sciences, medicine, and industrial production. Creative Proteomics offers post-translational modification analysis services for proteins and complies with the ICH Q6B guideline's standards for modification identification.
What is Post-Translational Modification (PTM)?
Post-translational modifications (PTMs) delineate the biochemical intricacies that transpire in a protein once its synthesis has been effectuated through translation by ribosomes. Such modifications possess the capacity to radically influence the protein's configuration, stability, function, locale, and their ability to interact, thereby serving as a cornerstone in cellular function and regulation.
A multitude of amino acids within protein sequences may undergo PTMs, incorporating but not confined to phosphorylation, methylation, acetylation, glycosylation, ubiquitination, and hydroxylation. Different PTMs breed variant effects on protein function. To illustrate, the processes of phosphorylation and dephosphorylation on specific amino acids can proclaim dominion over protein activity through the addition or removal of phosphate groups. Both methylation and acetylation can engender modifications in lysine or arginine residues, subsequently impacting protein-protein interactions or gene expression regulation.
Fig 1. Protein modifications in bacteria. (Macek, B., et al.; 2019)
PTMs are fundamental to numerous biological processes, which include cell signaling, gene expression, protein degradation, and protein trafficking. They imbue an added dimension of regulation, empowering proteins with the ability to dynamically adapt to cellular prompts or environmental shifts. It has been observed that dysregulation or aberrations in PTMs can contribute to a range of human diseases, housing cancer, neurodegenerative diseases, and metabolic disorders, among others. Thus, the study of PTMs in terms of discourse and investigation amplifies our comprehension of protein function, cellular operations, and the mechanistic basis of diseases.
Application of Post-Translational Modification (PTM)
Regulation of protein function: PTMs perform instrumental roles in the modulation of protein function, including activity, stability, localization, and molecular interactions. This modification allows cells to fine-tune protein functions in response to stimuli, both internal and external. Given the pivotal role that protein regulation plays in cell biology, PTMs are vital for a slew of cell functions ranging from signal transduction, metabolic function to gene expression, and cell cycle control.
Enzyme regulation: In the domain of enzyme regulation, PTMs have profound implications as modifiers of enzymatic performance, either activating or deactivating enzyme catalytic proficiency. In certain cases, enzyme phosphorylation can either upregulate or subside their activity, leading to a significant impact on cellular signaling cascades or metabolic pathways.
Protein degradation: The role of PTMs extends to guiding protein degradation. PTMs have the ability to earmark proteins for destruction by annotating them with dedicated modifications. Ubiquitination, for example, labels proteins for proteasomal degradation which facilitates the elimination of impaired, or superfluous proteins from the cell.
Cell signaling: Given the critical part PTMs play in cellular signaling cascades, they feature heavily in cell communication pathways. PTMs such as phosphorylation, acetylation, glycosylation, and methylation are capable of switching proteins ‘on’ or ‘off’ in cell signaling networks. Such modifications allow cells to interpret and respond to extracellular signals by orchestrating appropriate internal responses.
Disease biomarkers: PTMs are seldom detached from disease pathology. Altered PTM pathways have been linked to a broad spectrum of diseases including neurodegenerative disorders, metabolic diseases, and cancer. Recognizing and examining PTMs can offer significant insights into disease biomarkers, paving the way towards enhanced disease diagnosis, prognosis, and identification of potential therapeutic targets.
Drug development: Gaining an understanding of PTMs, including their functional repercussions, can inform targeted therapy design and development. Thus, adjusting specific PTMs can pave the way for protein function modulation, the reinstatement of normal cellular functionality, and could even present potential therapeutic strategies for disease treatment.
Our Services
The evaluation of protein glycosylation represents a crucial dimension of biological research and investigation. Our team offers accurate and comprehensive protein glycosylation analysis, thereby offering a profound comprehension of protein structure and function. This service distinctly positions our collaborators to contribute quintessentially to the domains of scientific research and drug discovery. We believe this endeavor is not just the study of proteins, but rather, understanding life's blueprint at a deeper, molecular level.
Creative Proteomics, a premier company known for its substantial proficiency in advanced proteomics services, provides a highly customized N-linked glycosylation analysis service to valued clients. We leverage our team of seasoned experts and cutting-edge facilities and methods to analyze and identify N-linked glycan modifications in proteins—an important facet of biochemistry and molecular biology that influences protein function, stability, and intracellular processing. Our wide-ranging service empowers clients ranging from academic researchers to pharmaceutical industry professionals to enhance their understanding of the glycosylation process and its associated influences.
Creative Proteomics is an industry-leading specialist proteomics organization that provides impeccable O-linked glycosylation services. This mechanism occurs primarily in the intracellular Golgi apparatus and results in significant changes in the structure and function of proteins. In executing this process, Creative Proteomics utilizes state-of-the-art technology, equipment, and a team of highly skilled professionals to provide invaluable assistance to researchers and pharmaceutical companies in not only optimizing protein synthesis, but also facilitating a deeper understanding of protein function. These services are part of Creative Proteomics' comprehensive proteomics offerings, all of which are carefully tailored to facilitate scientific breakthroughs and advances in molecular biology.
Creative Proteomics, a globally recognized institution for its leading-edge proteomics research technologies, has announced the provision of an advanced glycosylation site occupancy analysis service. This premier service utilizes state-of-the-art methodologies for analyzing glycosylation-specific sites on proteins and elucidating the positional occupancy of each glycosylation site. Such a detailed analysis is paramount for gaining a thorough understanding of protein functions, their roles in multiple cellular processes, and their involvement in disease pathology. By offering the glycosylation site occupancy analysis service, Creative Proteomics seeks to streamline research endeavors in the biomedical space by empowering scientists and researchers with pivotal biological insights into glycosylation.
Creative Proteomics, a renowned biotechnology firm specializing in diverse scientific research solutions, prominently provides a robust and trustworthy Glycopeptides Analysis Service. This service is specifically designed to deliver exhaustive and precise analysis of glycopeptides, thereby potentially aiding researchers to decipher protein's function, structure, and interplay. By leveraging cutting-edge technology and sophisticated equipment, our highly experienced team is proficient in conducting thorough glycopeptide analyses and experiments. The essence of the glycopeptides analysis service from Creative Proteomics resides in the firm's solid commitment towards supporting researchers and scientists, enabling them to attain profound revelations in the realm of proteomics.
Protein acetylation analysis service offered by Creative Proteomics is carefully constructed intending to support fellow researchers and scientists in intricately examining the acetylation of proteins in disparate samples. Leveraging groundbreaking techniques such as mass spectrometry, the service promises precise identification and quantification of acetylation sites. In the larger perspective, it significantly contributes to the progressive strands of proteomics and drug discovery. The team of experts at Creative Proteomics pledges to deliver exact, trustworthy, and reproducible findings, assigning the Protein Acetylation Analysis Service as an exceptional resource for your investigative endeavors.
In the field of proteomics and its related areas, Creative Proteomics provides an extensive service for Protein Phosphorylation Analysis. This service is meticulously crafted to assist researchers in the identification, quantification, and examination of different phosphorylation sites in various proteins. By employing high-throughput phosphoproteomics analysis technology, Creative Proteomics is able to detect and analyze phosphorylated proteins with exceptional sensitivity and precision. This advanced detection methodology significantly reduces false positive rates leading to improved credibility in data analysis.
At Creative Proteomics, we lead in the provision of advanced proteomic services and now introduce our specialized Protein Ubiquitination Analysis Service. This high-precision service provides extensive ubiquitination analysis for diverse biological specimens. We utilize avant-garde technologies and methodologies with a focus on identifying and quantifying ubiquitination sites, pivotal to cellular processes. Our rigorous quality control system, together with a robust data analysis pipeline, guarantees highly dependable experimental outcome. Beyond doubt, our service proffers profound insights into the intricate dynamics of protein ubiquitination which is a key contributor in comprehending and researching cellular processes and diseases.
The protein deamidation analysis service offered by Creative Proteomics represents a valuable tool for fellow researchers and investigators in our field. Protein deamidation, a crucial post-translational modification, frequently plays a pivotal role in affecting protein stability, topological structure, and functional characteristics—potentially influencing a range of subsequent biological phenomena. With comprehensive laboratory facilities at their disposal, our team can perform exhaustive analysis on a broad scope of sample types, ranging from cell lysates to purified proteins. Creative Proteomics is well-equipped to accommodate demands from diverse sectors, such as biopharmaceutical entities, academic institutions, and healthcare research organizations.
Creative Proteomics is a prominent organization that excels in delivering proteomics services of the highest standards. Our protein oxidation analysis service is an exceptional stride in the field, employing groundbreaking techniques and novel methodologies to meticulously determine oxidative protein modifications. Our highly specialized service plays a pivotal role in research related to oxidative stress, aging, and various pathologies that center around oxidative impairments. Creative Proteomics' protein oxidation analysis service is meticulously devised to detect and accurately measure protein oxidation levels, which could precipitate alterations in the protein's function and structure.
As a prominent enterprise in the realms of proteomics and genomics, Creative Proteomics offers an extensive protein methylation analysis service. This avant-garde service is engineered with the capacity to detect, recognize, and measure protein methylation. Our committed team, composed of experienced scientists and researchers, harnesses cutting-edge modalities such as mass spectrometry to deliver our clients precise, high-definition detection and evaluation of protein methylation.
As a leading company specialized in research, Creative Proteomics offers advanced protein alkylation analysis services. Our service caters to researchers in the scientific community studying the effects and implications of protein alkylation in different biological processes. Our team of professional technicians utilize state-of-the-art technology and methodologies to carry out comprehensive study on protein alkylation, guaranteeing accurate and dependable results delivered in a timely manner. Our services are applicable across a diverse range of research sectors, including pharmacology, biochemistry, molecular biology, and more. Our protein alkylation analysis service allows researchers to direct more attention towards their core research, trusting the complex task of protein alkylation analysis to our seasoned experts.
As a prominent entity in the domain of advanced proteomics services, Creative Proteomics provides an extremely specialized service known as protein sulfation analysis. This highly specialized service deals with the investigation and assessment of protein sulfation, a crucial post-translational modification imperative to various cellular functions. This offering is particularly advantageous for scientists and researchers necessitating meticulous details regarding the sulfation status of certain proteins. The protein sulfation analysis service commits to delivering comprehensive, high-quality reports, facilitating an in-depth understanding of the functionality and interaction of sulfated proteins within biological systems.
Creative Proteomics, a reputable provider of various types of proteomics services, offers a special service known as proteolysis analysis service. This service is indispensable for comprehending the biological significance of proteins, their correlation with diverse pathologies, and their interaction dynamics with pharmaceutical compounds. Proteolysis is a pivotal process where proteins undergo a simplification transformation via enzymatic or non-enzymatic pathways.
Creative Proteomics scientifically leads in the provision of an array of proteomics services, paramount among them being our unique protein truncation analysis service. This distinct service is instrumental in investigative research in relation to protein structure and functionality. The protein truncation analysis service empowers researchers to conduct systematic examination of the implications of truncations on the efficiency of varying proteins, thus providing insights into the varying roles and operations of different protein domains. Our service incorporates detailed truncation of peptides at precise points, afterwards performing meticulous analysis via advanced methodological scientific instruments and approaches.
Sample Requirement
| Sample Type |
Protein |
Cell |
Animal tissue |
Plant Tissue |
Blood (EDTA added) |
Serum |
Urine |
Microbes |
| Quantity |
100 ug |
2X107 cells |
1g |
200 mg |
1mL |
0.2-0.5 mL |
2 mL |
Dry weighed:
200 mg |
Service Process
Our Goals
As a reputable CRO, Creative Proteomics is dedicated to assisting our clients in meeting the rising need for protein drug characterization on a global scale and is assured of offering services of the highest caliber. Please don't hesitate to get in touch with us if you're interested in our services or have any other inquiries.
Reference
- Macek, B., et al.; Protein post-translational modifications in bacteria. Nature Reviews Microbiology. 2019
Related Sections
Services
Applications
For research use only, not intended for any clinical use.
