Glycopeptide Microarray Service

What is Glycopeptide Microarray?

A glycopeptide microarray is a high-throughput screening platform used in the field of glycobiology to study the interactions between glycoproteins and other molecules. Glycoproteins are proteins that have sugar molecules attached to them, and they play crucial roles in various biological processes, such as cell signaling, immune response, and disease progression.

In a glycopeptide microarray, small spots containing different glycopeptides are immobilized onto a solid support, such as a glass slide or a membrane. These glycopeptides may represent various glycoproteins or glycan structures. We can then expose the microarray to different molecules, such as proteins, antibodies, or small molecules, to analyze their interactions with the immobilized glycopeptides.

The binding interactions between the molecules and the glycopeptides on the microarray can be detected and quantified using various methods, such as fluorescence, mass spectrometry, or surface plasmon resonance (SPR). By analyzing the binding patterns, we can gain insights into the specificity and affinity of molecular interactions involving glycoproteins, which can be valuable for understanding biological processes and developing new diagnostic or therapeutic strategies. Glycopeptide microarrays have applications in areas such as drug discovery, biomarker identification, and vaccine development.

Glycopeptide Microarray Platform Offered by Creative Proteomics

Comprehensive Glycan Profiling: This platform facilitates comprehensive profiling of glycan structures within complex biological samples. By immobilizing diverse glycopeptides on the array, users can systematically interrogate the glycan composition of proteins, cells, tissues, and bodily fluids with high sensitivity and specificity.

Glycan-Protein Interaction Studies: The platform enables in-depth investigation of glycan-protein interactions, shedding light on the molecular mechanisms underlying various biological processes. Users can characterize the binding affinities and specificities of glycoproteins with a wide array of glycans, elucidating their roles in cellular signaling, immune recognition, and disease pathogenesis.

Biomarker Discovery and Validation: With the glycopeptide microarray platform, users can accelerate the discovery and validation of novel glycan-based biomarkers for disease diagnosis, prognosis, and therapeutic monitoring. By analyzing glycan alterations associated with different disease states, valuable insights into disease mechanisms and potential therapeutic targets can be gained.

Drug Target Identification and Validation: The platform serves as a valuable tool for drug discovery and development by facilitating the identification and validation of glycan-mediated drug targets. Through systematic screening of potential drug candidates against glycopeptide arrays, valuable insights into drug-glycan interactions can be obtained, aiding in the selection of promising drug candidates.

Structural and Functional Characterization of Glycoproteins: The glycopeptide microarray platform enables detailed structural and functional characterization of glycoproteins, offering insights into their glycan heterogeneity, post-translational modifications, and biological activities. By analyzing the interactions between glycoproteins and specific glycan motifs, valuable information about their roles in cellular processes and disease pathways can be obtained.

Workflow of Glycopeptide Microarray Services

a) Sample Preparation

• Clients provide biological samples for glycopeptide analysis.
• We conduct sample extraction, purification, and enzymatic digestion if necessary to obtain glycopeptides of interest.

b) Array Design and Fabrication

• Custom design of glycopeptide microarrays based on client specifications and research goals.
• Synthesis or procurement of a diverse library of glycopeptides representing various glycan structures and protein targets.

c) Immobilization onto Solid Support

• Immobilization of synthesized glycopeptides onto a solid support using appropriate chemistries.
• Ensuring uniform distribution and optimal orientation of glycopeptides for enhanced binding interactions.

d) Blocking and Stabilization

• Blocking of non-specific binding sites on the array surface to minimize background signal.
• Stabilization of immobilized glycopeptides to maintain structural integrity and functionality.

e) Sample Incubation and Binding Assays

• Incubation of prepared glycopeptide microarrays with client samples to initiate binding interactions.
• Conducting binding assays under controlled conditions to ensure reproducibility and accuracy.

f) Washing and Detection

• Washing of microarrays to remove unbound or non-specifically bound molecules.
• Utilization of appropriate detection methods for visualization and quantification of binding events.

g) Data Acquisition and Analysis

• Image capture of microarrays followed by data analysis to identify binding patterns and quantify affinities.
• Utilization of bioinformatics tools for data processing, normalization, and statistical analysis.

h) Result Interpretation and Reporting

• Interpretation of experimental results in the context of client's research objectives and scientific literature.
• Generation of comprehensive reports summarizing key findings and potential implications.

i) Consultation and Follow-Up

• Provision of consultation services to address client inquiries and discuss results.
• Ongoing support and collaboration opportunities to foster continued scientific advancement.

Advantages of Glycopeptide Microarray

Sample Requirements for Glycopeptide Microarrays