Single glycan quantitative analysis entails the precise quantification of individual glycan molecules within a complex biological sample. Unlike conventional glycomics approaches that focus on the overall glycan composition or structural profiling, single glycan quantitative analysis zooms in on the quantitative assessment of individual glycan species present in a sample. This granular approach enables researchers to decipher the subtle variations in glycan abundance across different biological contexts, shedding light on the intricate roles that glycans play in various physiological processes and disease states.
At Creative Proteomics, we are dedicatedly contributing to the advancements in this field with our precise and reliable single glycan quantitative analysis services. Our unique blend of cutting-edge analytical tools and experienced professionals guarantee results that will profoundly impact your research progression.
Technical Platform for Single Glycan Quantitative Analysis
Liquid Chromatography-Mass Spectrometry (LC-MS):
Creative Proteomics harnesses LC-MS platforms, such as the Thermo Scientific™ Orbitrap™ series and the Agilent 1290 Infinity II LC system coupled with Triple Quadrupole Mass Spectrometry, for high-throughput glycan analysis. LC-MS enables the separation, detection, and quantitation of individual glycans based on their mass-to-charge ratio (m/z), offering exceptional sensitivity and dynamic range for complex sample analysis.
Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS):
We employ MALDI-MS instrumentation, including the Bruker Daltonics ultrafleXtreme MALDI-TOF/TOF mass spectrometer, for rapid and label-free glycan analysis. MALDI-MS facilitates the direct ionization of glycans from solid substrates, enabling fast acquisition of mass spectra with minimal sample preparation. This technique is particularly well-suited for high-throughput glycan profiling and imaging applications, offering insights into spatial distribution patterns within biological tissues and cellular samples.
Single Glycan Quantitative Analysis Solutions at Creative Proteomics
Glycan Profiling and Structural Characterization – A broad service that includes the identification, structure elucidation, and quantitative assessment of individual glycans in a sample.
Glycan Sequencing – Utilizes state-of-the-art techniques to sequence glycans for structural identification and analysis.
Glycan Biomarker Discovery – Pioneering approach to find specific glycan markers related to diseases.
Glycoprotein Analysis – Comprehensive analysis of glycoproteins including both protein and glycan portions.
Sample Requirements for Single Glycan Quantitative Analysis
| Sample Type | Recommended Amount |
|---|---|
| Serum/Plasma | 100-500 µL |
| Tissue Homogenate | 10-50 mg |
| Cell Lysate | 1-5 x 106 cells |
| Urine | 1-5 mL |
| Saliva | 0.5-2 mL |
| Cerebrospinal Fluid | 0.5-1 mL |
| Milk | 1-10 mL |
| Food Samples | Variable |
| Biofluids (e.g., tears, sweat) | Variable |
Choose Single Glycan Analysis or Total Glycan Analysis?
| Criteria | Single Glycan Analysis | Total Glycan Analysis |
|---|---|---|
| Focus | Structure and properties of individual glycans | Overall composition and dynamics of the glycan pool |
| Objective | Deeper understanding of specific glycan behaviors and functions | Assessment of the global glycan profile and dynamics |
| Applications | Identify unknown glycans, characterize anomalous structures, analyze nano-scale interactions | Determine overall glycan profile, monitor global changes due to disease or conditions, establish correlations with biological phenotypes |
| Approach | Isolate and analyze individual glycans | Assess the collective glycan composition and dynamics |
| Complexity | High technical complexity, resource-intensive | Lower technical complexity, less resource-intensive |
| Suitability | Studies focusing on individual glycan properties and functions | Systemic investigations requiring an overview of the glycan landscape |
| Integration | Can be integrated with Total Glycan Analysis for comprehensive insight | Provides foundational data for subsequent Single Glycan Analysis |