Fluorescent Neutralization Test
Antibody titers against a viral pathogen can be measured by neutralization assays. This assay is a specialized type of immunoassay and detects the presence of virus-neutralizing antibodies that are capable of inhibiting virus replication in a sample. To date, the neutralization assays include the traditional, such as the plaque reduction neutralization test (PRNT) and modern method, such as fluorescence reduction neutralization assay (FRNA). Creative Proteomics is committed to becoming one of the leading suppliers in the field of virus detection and identification service. Here we provide FRNA assay to meet the need for reliable and rapid measurement of neutralizing antibodies of our customers in pre-clinical trials. Our neutralization assay platform is based on advanced instruments and highly experienced scientists. Therefore, we are capable of achieving worldwide customers' research goals with the best strategies and service.
Virus neutralization test
For determination of antibody efficacy in many virus-caused diseases, virus neutralization tests are the gold standard. Among them, plaque reduction neutralization test (PRNT), which is based on plating virus-antibody mixture on virus-susceptible cells to detect and measure neutralizing antibodies, is the classical and most widely used method. However, the method is laborious and time-consuming due to its requirement of manual counting of plaques and immunostaining. Meanwhile, the operator bias and low throughput make it less suitable for large scale serodiagnosis and vaccine evaluation. Fluorescence reduction neutralization assay (FRNA) is an alternative to PRNT. Based on fluorescence measurement and analysis, the method can investigate neutralization of viruses by antibodies in an easier and faster way.
Fluorescent neutralization test in Creative Proteomics
We have a complete virus culture and isolation system, including cell culture facilities and special laboratories. As for conducting FRNA assay, based on advanced instruments, such as the fluorescence reader, each plate can be completed in several minutes, involving reading, counting and analysis. The automated counting system not only achieves a faster and more efficient assay by computerized image scanning and analysis, but is also capable of differentiating plaques of different morphology. Besides, thanks to the employment of robotic automation of plates, our service can access high throughput detection.
The flowchart of FRNA assay.
Benefits of our service
- One-stop solution, ranging from cell culture to final reports delivery.
- Fast and accurate virus neutralization test.
- The most competitive price.
For virus neutralization test, Creative Proteomics' automated assay system makes FRNA more suitable for standardization and high throughput purposes with improved turnaround times. With a focus on providing high-quality service and passionate about our customers, we are your reliable and professional partner in the field of virus detection and identification. If you are interested in our services, please contact us for more information.
References
- Postnikova, E. N., et al. (2019). "Scalable, semi-automated fluorescence reduction neutralization assay for qualitative assessment of Ebola virus-neutralizing antibodies in human clinical samples." PloS one, 14(8), e0221407.
- Muruato, A. E., et al. (2020). "A high-throughput neutralizing antibody assay for COVID-19 diagnosis and vaccine evaluation." Nature communications, 11(1), 1-6.
* For research use only.
