Microarray-Based Virus Detection Service
DNA microarray is a genome-based method, also called microarray or DNA chip, which is characteristics by high sensitivity and specificity. With the advances in microarray technologies, new platforms that are being widely adopted for detection and identification of viral targets have been established. Since microarrays allow multiplexing and having the capability to test for multiple targets simultaneously from the same specimen, the methods are well suited for identification of the virus. As a leading biotechnology company, Creative Proteomics provides microarray-based viral detection service to satisfy research goals of worldwide customers.
DNA microarray-based detection
As an unconventional molecular methodology, microarrays have been applied to microbial detection, genotyping, and antimicrobial resistance gene detection. The technology is made up of probes of oligonucleotides. The probes designed for diagnostic assays are characteristics by high density and unique to a specific pathogen with respect to the other pathogen genomes as well as host other nonspecific genome sequences in the samples. The microarray-based viral detection started at the beginning of this century. With the first applied for detection the then unknown SARS coronavirus, microarrays based on oligonucleotide probes now have been widely used for detection of many viruses. Particularly, the technique has been widely used for identifying viruses in livestock animals and has become one of the most recent diagnostics in veterinary field. As flexibility and high-throughput capability and other potential advantages, the method has advanced biological sciences profoundly.
DNA microarray-based viral detection services in Creative Proteomics
Creative Proteomics is a reliable partner. We are aimed at serving world customers with first-class service experience, optimal solutions and professional technical support. With our extensive experienced experts and advanced platform, we provide an integrated portfolio of DNA microarray-based viral detection services, including probe designing for viral detection, sample processing, microarray and data processing. Additionally, we also offer clients with a variety of customized services to meet your special needs.
Probe designing for viral detection
Probes are the most important constituent of a microarray chip. With advanced platform, we are capable to provide our customers a custom DNA microarray for low input detection and identification of viruses. Each virus species on the array is represented by sense oligonucleotide probes whose sequences are based on the specific viral genomes.
Sample processing
To accommodate low input levels of viral targets, we have developed optimized protocols for sample processing, including a carrier cDNA, RNase treatment and optimization of DNase I-mediated target fragmentation.
Microarray and data processing
DNA microarray-based viral detection uses virus-specific oligos immobilized on a membrane as probe and specially designed software for data analysis. The microarray processing is under a standard and rigorous procedure, including target hybridization, post-hybridization washing, staining and scanning. In addition, we employ alternative data analysis methods to distinguish the genotypes of the viruses. Besides, to identify a minority species in a multi-virus complex, the single probe-based analysis approach is applied.
The workflow of DNA microarray-based viral detection service
Reference
- Pokhriyal M., et al. (2019). "Bioinformatics and Microarray-Based Technologies to Viral Genome Sequence Analysis." Microbial Genomics in Sustainable Agroecosystems. Springer, Singapore.115-129.
* For research use only.
