Integrated Transcriptomics and Proteomics Analyses
Transcriptomics and proteomics are important tools to obtain gene expression. From the biological perspective, transcriptome represents the intermediate state of gene expression and can reflect the mechanisms such as transcriptional regulation and post-transcriptional regulation. Proteome is all the proteins that an organism can express. Proteins are the direct functional executors of the organism. Protein changes play an important role in the growth of organisms, environmental stress, the occurrence, and development of diseases. In order to accurately explore the molecular mechanism of viral infection and comprehensively understand virus-host interaction, proteome and transcriptome datasets should be integrated to construct a virus-host interaction network. The study of virus-host interaction helps to identify the host cell network affected by the virus and the host factors required for virus replication, promoting the research and development of novel antiviral drugs.
Integrated transcriptomics and proteomics analyses
Our transcriptome profiling services help clients analyze ribonucleic acid (RNA) transcripts and gain a deep understanding of gene expression patterns and gene regulation mechanisms. Our experienced scientists use advanced platforms to perform RNA-sequencing studies, non-coding RNA analysis, among others.
Nowadays, proteomics has emerged as a promising experimental tool for global profiling has made significant technological advances. MS-based proteomic platforms have been successfully used to better understand the mechanisms of viral infection. The study of viral proteome can provide essential understanding from the identification of entry receptors to altered host-cell pathways, expediting the investigation of disease pathogenesis and the identification of potential antiviral druggable targets. We provide the most common quantitative proteomic techniques, including isobaric tags for relative and absolute quantification (iTRAQ)-based proteomic analysis, tandem mass tag (TMT)-based proteomic analysis, stable isotopic labeling with amino acids in cell culture (SILAC)-based proteomic analysis, and label-free quantitative proteomic analysis.
The workflow of our services
Integrated transcriptomics and proteomics analyses are a powerful tool for building a picture of a new virus because of its ability to accurately outline transcriptional profiles and add important new directly observed data to viral proteins. As the machinery of science continues to turn, Creative Proteomics is committed to keeping pace. We have established an analysis platform combining transcriptomics and proteomics and accumulated rich experience in related experimental design and analysis. Our technology platform is designed according to the pathogenesis of the disease, the different parts of the sample, the total amount of samples, and other characteristics. The general workflow for integrated transcriptomics and proteomics analyses is as follows,
Technological advantages
- Establish a protein database and greatly increase the number of protein identification
- Multi-group cross-validation: an in-depth exploration of the regulation mechanism
- The gene mutation information is verified at the protein level
An integrative proteome and transcriptome analysis can provide direct observation of transcripts, proteins, and potential phosphorylation sites. Critically, the joint analysis on the virus-host interaction provides a comprehensive overview of these host factors and suggests novel alternatives of these host factors as drug targets. Creative Proteomics is proud to offer viral multi-omics analysis services to advance virus research. We are committed to providing high-quality and customized service to greatly accelerate our customers' project progress and success. Feel free to contact us with any questions about our services. We will be delighted to provide you with all the support we can to help establish a sustaining professional partnership.
* For research use only.
