Viral Long-Read RNA Sequencing Service
Long-read Seq (LRS) technologies allow for the precise characterization of full-length transcripts, providing enabled new methods for studying the transcriptome and its functions. LRS can sensitively identify new transcripts, transcription subtypes, and transcription overlaps, and contribute to the kinetic characterization of the viral transcripts. LRS is well suited to characterize the smaller transcriptome of organisms with known reference genomes and reveals a more complex viral transcriptome. Creative Proteomics is a good partner of pharmaceutical companies and research institutions. Here, we provide a robust viral RNA sequencing using long-read sequencing to provide new insights into the study of viruses and their effect on host gene expression.
Long-read sequencing (LRS)
The current iteration of LRS allows the sequencing of polyadenylated mRNAs from the 3' poly(A) tail toward the 5' cap. As a third-generation approach, LRS has revolutionized genomics and transcriptomics. This approach has a relatively low throughput compared to Illumina sequencing (short-read sequencing), but it addresses the challenges of earlier technologies by obviating the need for computational splicing of sequence fragments together to reconstruct the original transcript. Transcriptome surveys based on LRS have demonstrated the potential of this new technology in identifying novel transcripts as well as characterizing transcript isoforms. At present, there are two most widely used LRS technologies, including Pacific Creative Proteomics' (PacBio) single-molecule real-time (SMRT) sequencing and Oxford Nanopore Technologies' (ONT) nanopore sequencing. The read lengths obtained by SMRT and nanopore sequencing surpass the lengths of most transcripts, which are about 15 kb and > 30 kb respectively. In addition, nanopore sequencing allows RNA to be sequenced directly.
Service offering in Creative Proteomics
LRS can accurately characterize full-length transcripts, making it an indispensable tool in transcriptomics. Based on Pacific Creative Proteomics (single molecule real-time sequencing, SMRT) and Oxford Nanopore (Nanopore sequencing) technologies and instruments, we have launched LRS platforms for studying the transcriptome complexity of viruses. Through cDNA and direct RNA sequencing analyses, we are able to investigate the extremely complex transcriptional landscape of a variety of viruses. Our expertise includes infection, sequencing as well as data integration strategy for transcript annotation.
Fig1. Viral RNA sequencing workflow using LRS technologies.
LRS techniques can detect more transcript overlaps, polycistronic RNAs, splice, and transcript variants, overcoming the challenges of short-read sequencing. As a result, LRS have been applied to study the transcriptomes of a variety of viruses (such as baculoviruses, herpesviruses, and poxviruses) and provide a more complexity of viral transcriptomes than earlier approaches. Creative Proteomics' experienced team can provide genomics, transcriptomics, proteomics, metabolomics, and lipidomics experiments as well as in-depth multi-omics data analysis services, greatly accelerating global customers' project progress. Our success lies in constantly optimizing our services. Whatever the scale and complexity of your project for a professional, please feel free to contact us for reliable and highly customized solutions.
References
- Oikonomopoulos, S., et al. (2020). "Methodologies for transcript profiling using long-read technologies." Frontiers in genetics, 11, 606.
- Tombácz, D., et al. (2020). "Long-read Assays Shed New Light on the Transcriptome Complexity of a Viral Pathogen and on Virus-Host Interaction." bioRxiv.
* For research use only.
