Viral SMRT full-length RNA-seq Service
At present, viral transcriptome studies are generally performed using Illumina sequencing, including the discovery and precise annotation of viral transcripts. Creative Proteomics has the capability to assist global customers with the most challenging viral transcriptomics sequencing projects. Based on the advanced PacBio single molecular real-time (SMRT) sequencing platform, the transcript isoforms (such as length- and splice variants) and overlapping monocistronic/polycistronic RNA molecules can be identified. Our experienced and professional team can provide comprehensive and reliable service for a wider range of viral transcriptome analyses.
SMRT sequencing for viral transcriptomic analysis
As one of the long-read sequencing platforms, single-molecule real-time (SMRT) sequencing is a proven technology and widely used in various fields of life science in recent years, such as metagenome analyses of viruses or bacteria, transcriptome analyses of splicing variants, and determination of the full-length genome sequences of bacteria and so on. SMRT sequencing is a sequencing-by-synthesis technology that enables the direct, real-time observation of DNA synthesis by DNA polymerase. Since the reaction is driven by a DNA polymerase, and in this technique individual molecules are imaged, the signal does not degrade over time. The sequencing reaction does not end until the template and polymerase are separated. So far, SMRT sequencing produced an average sequencing read length of about 15 kb, with the longest sequencing read length exceeding 50 kb, about 200 times that of conventional NGS. SMRT sequencing is characterized by long reads, high consensus accuracy, a low degree of bias, and simultaneous detection of epigenetic modifications. Therefore, it has been recognized by many researchers.
Understanding the full transcriptional repertoire of the virus is crucial for studying its gene expression. Our SMRT-based viral transcriptomic analysis can be used in the processes of viral replication, providing a dynamic transcriptome of viruses. Integrating this data with information obtained by applying other methods could greatly improve our understanding of viruses. We put our heart and soul into supporting our global customers with competitive and professional services. Application of our services, including,
- Investigation of the dynamic transcriptome of viruses
- Monitoring the individual dynamics of transcripts isoforms
- Monitoring the individual dynamics of intron variants
- Monitoring the individual dynamics of overlapping RNAs
- Characterization of the kinetic properties of novel viral transcripts
Workflow of our service
Advantage of our service
- Performing viral transcriptomic analysis with the most widely used LRS technology
- Rich experience in dealing with viral RNA sequencing
- Provide workflows and analysis tools for RNA sequencing customized for your research
- Strictly keep confidential the client's project information and experimental data
Compared to cellular organisms, most viruses have a relatively small genome. However, the features make viruses excellent subjects for comprehensive analysis of the dynamic transcriptome using long-read sequencing. Creative Proteomics' experienced team can provide genomics, transcriptomics, proteomics, metabolomics, and lipidomics experiments as well as in-depth multi-omics data analysis services, greatly accelerating global customers' project progress. Our success lies in constantly optimizing our services. Whatever the scale and complexity of your project for a professional, please feel free to contact us for reliable and highly customized solutions.
Reference
- Takeda, H., et al. (2019). "Exploring the hepatitis C virus genome using single molecule real-time sequencing." World journal of gastroenterology, 25(32), 4661.
* For research use only.
